blot


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North·ern blot a·nal·y·sis

a procedure similar to the Southern blot analysis, used to separate and identify RNA fragments; typically through transferring (blotting) RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
[coined to distinguish it from eponymic Southern blot a.]

blot

(blot) a technique for transferring ionic solutes onto a nitrocellulose membrane, filter, or treated paper for analysis; also used to describe the substrate containing the transferred material. For specific techniques, see under technique.

blot

(blŏt)
n.
The Northern, Southern, or Western blot analyses.

blot

1 a technique for transferring electrophoretically separated components from a gel onto a nitrocellulose membrane, chemically treated paper, or filter for analysis. It is frequently used to analyze genetic material.
2 the substrate containing the transferred material. See also Northern blot test, Southern blot test, Western blot test.

blot

noun A nitrocellulose or nylon membrane bearing a biomolecule of interest—e.g., DNA, RNA or protein—transferred to the membrane from an electrophoretic gel by either osmosis or vacuum; after transferring the molecule of interest, the membrane is bathed in a solution containing a “mirror-image” of the molecule already on the membrane, producing a hybridisation.
 
verb To perform any of a number of similar techniques used to analyse biologic molecules. Mixtures of biomolecule—DNA, RNA or protein—fragments are separated by electrophoresis through a polymeric (agarose or polyacrylamide) gel; the separated components are then transferred to a membrane where they are immobilised, analysed and visualised by various techniques including antibody binding and DNA hybridisation.

Southern blot
A technique for identifying the presence or absence of a segment of DNA in a sample; the procedure begins by partial enzymatic digestion of nucleic acids, cutting the DNA at specific sites by any of a number of restriction endonucleases, each of which recognises and cuts at a 5- or 6-nucleotide sequence. For instance, HindIII, from H influenzae, cuts DNA at all sites bearing the nucleotide sequence A/AGCTT at the mirror image sites on the 2 DNA chains between the 2 adenines A/A, resulting in a mixture of DNA fragments of varying lengths measuring up to 30 kD, which is then electrophoresed in an agarose gel and transferred to a membrane. Because the DNA on the membrane is single stranded, it has a high affinity for its complementary strand. The final step in the blot is to radioactively “tag” the complementary DNA (Southern blot, after Dr E. Southern), RNA (Northern blot), protein (Western blot), DNA-protein hybridisation (Southwestern blot), and RNA-protein hybridisation (Northwestern blot).

Dot blot
DNA, RNA, or protein are dotted directly onto a membrane support, and form discrete spots.

North·ern blot a·nal·y·sis

(nōr'dhĕrn blot ă-nal'i-sis)
A procedure similar to the Southern blot analysis, used mostly to separate and identify RNA fragments; typically done through transferring RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
[coined to distinguish it from eponymic Southern blot a.]

South·ern blot a·nal·y·sis

(sŭdh'ĕrn blot ă-nal'i-sis)
A procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridized with complementary (labeled) nucleic acid probes.

West·ern blot a·nal·y·sis

(wes'tĕrn blot ă-nal'i-sis)
A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or post-tagged with a labeled secondary protein.
See also: immunoblot
Synonym(s): Western blot, Western blotting.
[coined to distinguish it from eponymic Southern blot a.]

zoo blot a·nal·y·sis

(zū blot ă-nal'i-sis)
A procedure using Southern blot analysis to test the ability of a nucleic acid probe from one species to hybridize with the DNA fragment of another species.
References in classic literature ?
but there was, though; I know it, because I rubbed my finger - this very one - in the powder that was over the blot.
Baisemeaux," said Aramis, "and whatever you may have seen, the order is signed to release Marchiali, blot or no blot.
The blot of smoke which indicated the Macedonia's position had reappeared.
The blot of smoke had suddenly grown larger, and it was very black.
No father had watched my infant days, no mother had blessed me with smiles and caresses; or if they had, all my past life was now a blot, a blind vacancy in which I distinguished nothing.
Western Blot protein expression and WES analysis CRO services offer a complete platform to conduct protein expression studies from start to finish.
As reported here, Western blot may prove to be a good tool for diagnosis.
The phospho-ERK signal was normalized against the corresponding total ERK signal from the same Western blot to obtain the phospho-ERK/ERK ratio.
By suggesting humorous avenues of interpretation for ink blots, Think Blots greeting cards provide unique, delightful and entertaining ways of expressing affection, attraction, concern and love; things we like to convey through greeting cards.
The presence of entire SGI1 was also confirmed by Southern blot of XbaI-digested genomic DNA with the p1-9 probe containing a 2-kb EcoRI insert.
The nonclonal cells of Zeocin-resistant transfectants served as a positive control in the Western blot analyses of bad proteins (Figure 1B, lane 9).
Cross-absorption tests, especially in conjunction with Western blot immunoassays, can be used to determine the rickettsia species involved, as reported in this case.