blot


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North·ern blot a·nal·y·sis

a procedure similar to the Southern blot analysis, used to separate and identify RNA fragments; typically through transferring (blotting) RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
[coined to distinguish it from eponymic Southern blot a.]

blot

(blot) a technique for transferring ionic solutes onto a nitrocellulose membrane, filter, or treated paper for analysis; also used to describe the substrate containing the transferred material. For specific techniques, see under technique.

blot

(blŏt)
n.
The Northern, Southern, or Western blot analyses.

blot

1 a technique for transferring electrophoretically separated components from a gel onto a nitrocellulose membrane, chemically treated paper, or filter for analysis. It is frequently used to analyze genetic material.
2 the substrate containing the transferred material. See also Northern blot test, Southern blot test, Western blot test.

blot

noun A nitrocellulose or nylon membrane bearing a biomolecule of interest—e.g., DNA, RNA or protein—transferred to the membrane from an electrophoretic gel by either osmosis or vacuum; after transferring the molecule of interest, the membrane is bathed in a solution containing a “mirror-image” of the molecule already on the membrane, producing a hybridisation.
 
verb To perform any of a number of similar techniques used to analyse biologic molecules. Mixtures of biomolecule—DNA, RNA or protein—fragments are separated by electrophoresis through a polymeric (agarose or polyacrylamide) gel; the separated components are then transferred to a membrane where they are immobilised, analysed and visualised by various techniques including antibody binding and DNA hybridisation.

Southern blot
A technique for identifying the presence or absence of a segment of DNA in a sample; the procedure begins by partial enzymatic digestion of nucleic acids, cutting the DNA at specific sites by any of a number of restriction endonucleases, each of which recognises and cuts at a 5- or 6-nucleotide sequence. For instance, HindIII, from H influenzae, cuts DNA at all sites bearing the nucleotide sequence A/AGCTT at the mirror image sites on the 2 DNA chains between the 2 adenines A/A, resulting in a mixture of DNA fragments of varying lengths measuring up to 30 kD, which is then electrophoresed in an agarose gel and transferred to a membrane. Because the DNA on the membrane is single stranded, it has a high affinity for its complementary strand. The final step in the blot is to radioactively “tag” the complementary DNA (Southern blot, after Dr E. Southern), RNA (Northern blot), protein (Western blot), DNA-protein hybridisation (Southwestern blot), and RNA-protein hybridisation (Northwestern blot).

Dot blot
DNA, RNA, or protein are dotted directly onto a membrane support, and form discrete spots.

North·ern blot a·nal·y·sis

(nōr'dhĕrn blot ă-nal'i-sis)
A procedure similar to the Southern blot analysis, used mostly to separate and identify RNA fragments; typically done through transferring RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe.
[coined to distinguish it from eponymic Southern blot a.]

South·ern blot a·nal·y·sis

(sŭdh'ĕrn blot ă-nal'i-sis)
A procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridized with complementary (labeled) nucleic acid probes.

West·ern blot a·nal·y·sis

(wes'tĕrn blot ă-nal'i-sis)
A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre- or post-tagged with a labeled secondary protein.
See also: immunoblot
Synonym(s): Western blot, Western blotting.
[coined to distinguish it from eponymic Southern blot a.]

zoo blot a·nal·y·sis

(zū blot ă-nal'i-sis)
A procedure using Southern blot analysis to test the ability of a nucleic acid probe from one species to hybridize with the DNA fragment of another species.
References in periodicals archive ?
Once this happens, confidence in western blot data will inevitably follow.
immunoglobulin G Western blot confirmatory test for anti-toxoplasma antibody detection.
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Supplemental testing can be performed on serum, plasma, and dried whole-blood spots using the Genetic Systems Western blot kit.
By the time she was discharged, repeat HIV serologic testing showed positive ELISA and Western blot with bands at p17, p24, p31, p66, gp120, and gp160.
The Western blot, Liska says, "is a little more complicated to do.
Diagnostic sensitivity of polymerase chain reaction and Southern blot hybridization for the detection of human papillomavirus DNA in biopsy specimens from cervical lesions.
The investigators initially thought that the GenBio dot blot test would provide much better specificity because it does not contain antigens to Osp A, the recombinant protein that is found in LYMErix and can result in false-positive test results.
This RPA/slot blot method was also used to detect IL-2 mRNA in different murine cell lines.
With the C-DiGit Blot Scanner, researchers now have an effective solution that is priced below capital expenditure thresholds.
This is where western blot technology excels as a common follow up diagnostic test, offering a 100% accurate result.