blood smear


Also found in: Dictionary, Thesaurus, Legal, Encyclopedia, Wikipedia.

blood smear

a blood test used to provide information concerning drugs and diseases that affect the morphology of red and white blood cells and to help diagnose certain congenital and acquired diseases. A properly performed and analyzed blood smear is the most informative of all hematologic tests, allowing examination of erythrocytes, platelets, and leukocytes.
Enlarge picture
PREPARATION OF BLOOD SMEAR
Enlarge picture
PREPARATION OF BLOOD SMEAR

blood smear

A drop of (anticoagulated) whole blood spread thinly on a glass microscope slide so that blood cell types can be examined, counted, and characterized. Synonym: peripheral blood smear

Procedure: The slide must be grease-free. It is cleaned with alcohol, rinsed in warm water, and wiped clean with a lint-free towel or lens paper.A small drop of blood is placed on the slide; the end of another slide (spreader slide) is placed against the first slide at a 45° angle and pulled back against the drop of blood so that the drop spreads between the point of contact of the two slides. Then the spreader slide is pushed forward against the first slide; the blood will form an even, thin smear. The slide is dried by waving it in the air; it should not be heated. The blood smear is covered with Wright's stain and allowed to stand 2 min. An equal amount of distilled water or buffer solution is added and mixed uniformly. It is allowed to stand 5 min. The stain is gently washed off and the slide is allowed to dry.

See: illustrationillustration
See also: smear
References in periodicals archive ?
Malaria infection in pregnant women in south-eastern Nigeria using peripheral blood smear, placental blood smear and placental histology.
He also described a blood smear of a woman with an androgen-producing tumor of the adrenal gland, where 24% of the neutrophils had 1 or more "C" nuclear projections.
On average, 220 blood smears and 170 body fluid smears are reviewed monthly, which provides a substantial test volume on which to evaluate the clinical usefulness of the test.
A repeat blood smear on June 18 showed a parasitemia level of 0.
6 kg (<3rd percentile) (<3rd percentile) Height 118 cm 127 cm (<3rd percentile) (<3rd percentile) Weight for height (%) 107 97 Peripheral blood smear Neutrophil Neutrophil vacuolization vacuolization AST (U/L) (normal: 5-34) 43 113 ALT (U/L) (normal: 0-55) 63 192 CK (U/L) (normal: 30-200) 633 492 Abdominal ultrasound Grade 2 Hepatosplenomegaly hepatosteatosis and grade 2 hepatosteatosis AST: Aspartate aminotransferase; ALT: alanine aminotransferase; CK: creatine kinase.
Comparative study of peripheral blood smear, quantitative buffy coat and modified centrifuged blood smear in malaria diagnosis.
Treatment with suramin was started and repeat blood smears after 48 hours were clear of trypanosomes.
It is unclear, however, whether blood smear review is sensitive enough to be a practical diagnostic approach in potentially fungemic patients.
They fulfilled the criteria for HUS [hemolytic anemia with microangiopathic changes, typical deformation of erythrocytes in the peripheral blood smear (Burr cells), acute renal failure, and thrombocytopenia] and presented with pneumonia or meningitis.
The pipeline is designed to run on large series of images, such as in our study, where it was run on 104 images per blood smear.
Although automatic blood analyzers are widely used, peripheral blood smear is the hematological gold standard for definitive diagnosis.
Recent studies have demonstrated that sensitivity of blood smear examination performed by trained microscopists does not exceed 50% compared with PCR methods (9) and is much lower in field settings (9,10).