Transition from blastospores
to hyphae is an integral part of biofilm formation.
Liquid culture production of desiccation-tolerant blastospores
of the bioinsecticidal fungus Paecilomyces fumosoroseus.
and hyphae were counted by observation under a phase contrast microscope, according to the criteria described by Taweechaipapong et al.
Preparation, Deposition, and Viability
The researchers discovered that they could produce stable, effective blastospores
of Beauveria and Isaria in 2 to 3 days under appropriate LCF conditions with cottonseed flour medium.
and pseudohyphae in blood smears.
Control disks were handled identically, except that no blastospores
On the three types of media, the spores produced yeast-like blastospores
that continued to germinate, forming whitish, yeast-like colonies.
can be cultured for use in sprays to control leaf-feeding pests like aphids as easily as microsclerotia for use in granular formulations to control soilborne insects, like root maggots.
After the vehicle treatment, the control groups showed visible Candida blastospores
and pseudohyphae infiltrating epithelial cells and the number of visible microorganisms increased with time.
was 96% using the technique of Avery et al.
Hyphae result from the germination of blastospores
and are characterized by parallel walls at the point of germ tube emergence, absence of constrictions, and the development of true septa (Vale-Silva et al.