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biuret test

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biuret test
[bī′yoo͡ret]
Etymology: L, bis + Gk, ouron, urine
a method for detecting proteins in serum. In alkaline solution, copper sulfate ions react with the peptide bonds of proteins to produce a pink to purple color, called the biuret reaction. The amount of serum protein in a sample solution is estimated by comparing its color with that of a standard solution whose protein concentration is known.

biuret [bi´u-ret]
a urea derivative; its presence is detected after addition of sodium hydroxide and copper sulfate solutions by a pinkish-violet color (protein test) or a pink and finally a bluish color (urea test).
biuret reaction the reaction in biuret tests.
biuret test either of the tests done with biuret.


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[4] The relationship between edema, proteinuria, and the autopsy finding of abnormal kidneys was described in 1827 by Richard Bright The biuret test for protein was developed in 1833.
 
 
 
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