biotinylation


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biotinylation

The covalent linking of biotin to a protein, peptide, nucleic acid (DNA, RNA) or other biomolecule of interest, which in turn binds with high affinity to avidin or streptavidin linked to an enzymatic moiety or fluorescent molecule. Biotinylation is used in detector systems and to isolate biotinylated biomolecules of interest.
References in periodicals archive ?
1) Develop a novel tool to enrich early stage gametocytes from parasite culture using cell-type specific surface biotinylation and affinity purification.
Up to now, Creative Biomart has nearly six kinds of protein labeling service: stable Isotope Labeling, Protein Biotinylation, Fluorescent Labeling in vitro, Fluorescent Tagged Protein in vitro, Enzyme/Antibody conjugation and Site-specific labeling, the newly launched one.
Biotinylation of antibody 01D10 was performed by use of Sulfo NHS-LC-Biotin (Pierce) according to the manufacturer's instructions.
In vivo biotinylation demonstrates that reticulated platelets are the youngest platelets in circulation.
Using a cell surface biotinylation method, we found that the total cellular ABCA1 and that at the plasma membrane were increased with ceramide treatment.
1] gene (Detector[TM] PCR DNA Biotinylation Kit, KPL).
Selective analysis of phos-phopeptides within a protein mixture by chemical modification, reversible biotinylation and mass spectrometry.
During incubation, the anti-histone antibody bound to histone-competent apoptotic nucleosomes and linked the immunocomplex to the streptavidin-coated plates via its biotinylation.
For biotinylation, 1 mg of the purified ZP3 or Fetuin (taken as negative control in the study; Sigma-Aldrich Inc.
This innovative kit includes all the components for the rapid traceable biotinylation of a single antibody.
Topics include AMPA receptor trafficking, long-term plasticity at inhibitory synapses, proteomics of the PSD, phosphorylation of site-specific antibodies, protein palmitoylation by the DHHC protein family, antibody labeling and biotinylation approaches to study neurotransmitter receptors, visualization of AMPAR trafficking and surface expression, neurotransmitter dynamics, receptors dynamics and functional tagging, RNAi and applications in neurobiology, transfecting and transducing neurons, expression of recombinant proteins in rat brains using Sindbus virus, Lentivirus-based genetic manipulation, AMPA receptor phosphorylation in synaptic plasticity, and genomic and post-genomic tools for studying synapse biology.
Solulink Incorporated (San Diego, CA), the leader in the field of chemical biology, launched a unique quantitative biotinylation product, ChromaLink based on Solulink's innovative bioconjugation and immobilization technology.