bioluminescent reporter

bioluminescent reporter

A cell genetically engineered to emit light when introduced into a test animal. Reporter systems have made great contributions to the study of eukaryotic gene expression and regulation; they are most frequently used as indicators of transcriptional activity.

Examples
Bacteria, fungi, tumour cells.
References in periodicals archive ?
2005) or have used a bioluminescent reporter (such as luciferase) that has limited spatial resolution (Ciana et al.
Bagci-Onder will generate dual bioluminescent reporter systems of HRK and other TRAIL-associated factors, such as TRAIL receptors DR4/DR5; TRAIL signaling inhibitor proteins Flip, XIAP and Bcl-2 to assess gene expression changes.
Topics include staining and visualization of beta-galactosidase in mouse embryos and tissues, immunohistochemical detection of beta-galactosidase or green fluorescent protein on tissue sections, reporter gene expression in murine airways, 3D analysis of molecular signals with episcopic imaging techniques, fluorescent proteins for cell biology, detection of green fluorescent protein during nervous system developments in Drosophila melanogaster, autofluorescent proteins for flow cytometry, fluorescent protein reporter systems for single-cell measurements, subcellular imaging of cancer cells in live mice, noninvasive imaging of molecular events with bioluminescent reporter genes in living subjects and green fluorescent protein as a tracer in chimeric tissues.
The Promega luciferase reporters, specifically the luc2P construct, offer the best characteristics in a bioluminescent reporter assay.
Combined with the surface topography information, the software then analyzes and processes these images to determine the three-dimensional location and shape of the bioluminescent reporter.
Specifically, the software includes new tools that enable scientists to more accurately locate in three dimensions and quantify the signal strength of bioluminescent reporters, or glowing "tags" that light up targeted biological processes, in living animals.
All IVIS systems are optically capable of imaging the full spectrum of fluorescent and bioluminescent reporters and dyes, from green to near-infrared, with quantitative results and high performance spectral unmixing, to extract maximum information from animal models.
Promega is bringing its leading assay and detection technologies for bioluminescent reporters, cell viability, and P450-Glo substrate-based metabolism assays.
Coupled with the ability to provide 3D images of fluorescent and bioluminescent reporters, IVIS Spectrum is the first system to deliver these innovative capabilities in one instrument.