In one case (10%), positivity with 1 of those 2 primer sets was the only molecular evidence of biclonality; 2 clonal populations were identified with IGH FR1 and IGH FR3 primer sets in 4 cases (40%; 2 [20%] with IGH FR1 and 2 [20%] with IGH FR3), and 2 of these cases (the ones with biclonal
results for IGH FR1) also demonstrated biclonal
populations with the IGH FR2 primer set.
Tokla and Burnie, planted in the 1970s, contain some polyclonal or biclonal
The two bands of the biclonal
samples were separated and removed from the polyacrylamide gel and the bands were sequenced separately.
8 Serum IgG, mg/dL 2250 694-1618 24-Hour total urine protein, mg/24 h 983 40-150 Serum protein electrophoresis Biclonal
None IgG [lambda] Urine protein electrophoresis Free [lambda] None light chain Abbreviation: IgG, immunoglobulin G.
In addition, M-protein-containing specimens (IgG [lambda], IgA[kappa], and biclonal
IgMi[kappa] and IgG [lambda]) were treated similarly with either 100 mL/L ethanol or saline and held at 4 [degrees]C overnight.
Several PCR products were used to establish the method: a polyclonal, a clonal, a biclonal
, and two mixed-clonal products.
No cases of biclonal
or oligoclonal nodal MZL have been reported to our knowledge.
In addition, biclonal
gammopathies of different FLC types could produce normal [kappa]:[lambda] ratios although the concentrations of both molecules might be increased.
In addition, this technique is not useful when biclonal
or triclonal gatiitnopathies are seen with HRE.
Patients with known autoimmune diseases, in whom the MC was detected as part of the evaluation of their primary disease, were excluded from this study, as were patients with biclonal
In addition, two samples with biclonal
paraproteins were also evaluated.
In addition to monoclonal paraproteins, we also investigated four biclonal