Using state-of-the-art molecular cell biology including siRNA knockdown and reconstitution through bicistronic
retroviral mediated transfections, as well as genome editing we will generate stable cell lines expressing WT and functionally defined AP2 mutants.
subgenomic mRNA encodes both the ORF2 and ORF3 proteins of hepatitis E virus.
The human double sex-related gene, DMRT2, is homologous to a gene involved in somitogenesis and encodes a potential bicistronic
In a study of 12 patients with recurrent glioblastoma multiforme the therapeutic potential of combining HSVtk and IL-2 using a bicistronic Moloney vector, was reported using retroviral VPC (25,31).
Rather than using a bicistronic vector carrying both HSVtk and IL-2 genes, we have delivered HSVtk via retroviral VPC and IL-2 via direct intramuscular plasmid injection so that the IL-2 is in circulation longer (>10 days as reported earlier) (9).
As shown in Figure 2B, mice that are homozygous for the targeted insertion of the conditional rtTA-IRES-EGFP bicistronic
transgene at ROSA26 locus can be bred with double transgenic mice that carry a tissue-specific Cre transgene and a doxycycline-inducible rtTA-dependent tet-O-GENE.
This can be achieved by either using two separate plasmid constructs for transgenesis or using bicistronic
plasmids (Fu et al.
The expression of the first gene occurs by direct translation whereas the translation of the 5-distal gene(s) of bicistronic
(or polycistronic) mRNA will be promoted by IRESMP or IRESCP.
Equal transcription of wild-type and mutant p53 using bicistronic
vectors results in the wild-type phenotype.
As E6 and E7 are expressed together from a bicistronic
mRNA, such approaches generally lead to the reactivation of both pRb and p53, and to the attainment of a growth-arrested state that resembles the replicative senescence that is achieved by primary cells after they have reached their normal life span in culture.
Autonomous expression of the slo gene of the bicistronic
nga-slo operon of Streptococcuspyogenes.
Detection of the ORF3 polypeptide of feline calicivirus in infected cells and evidence for its expression from a single, functionally bicistronic
, subgenomic RNA.