DiscoveRx pioneered the use of beta-galactosidase
enzyme fragment complementation in biochemical and cell based assays for discovery research and holds extensive intellectual property in this area.
But when the cell lacks beta-galactosidase
-- the enzyme needed for normal breakdown and recycling of GM1 -- this lipid accumulates and causes gangliosidosis.
Like other PathHunter technologies, the Mitotic Index assays are engineered biosensor cell lines with separate nuclear and cytoplasmic fragments of Beta-galactosidase
Labels specifically claimed include green fluorescent protein (GFP), beta-galactosidase
, and luciferase.
The first grant is aimed at accelerating research into providing high throughput, homogeneous methods for G-Protein coupled receptor binding assays (GPCR) using the DiscoveRx core technology of complementation of inactive fragments of beta-galactosidase
as a signal amplification method.
Flow cytometry was utilized to rapidly assess the cell uptake efficiency and toxicity of fluorescence-labeled siRNA targeting the inhibition of beta-galactosidase
United States Navy -- entered into two Cooperative Research and Development Agreements (CRADAs) to evaluate the effectiveness of electroporation-mediated delivery of Luciferase, Beta-galactosidase
(B-gal) and human secreted embryonic alkaline phosphatase (SEAP) marker genes.
For the purpose of evaluating the effectiveness of gene delivery, the first agreement will investigate the electroporation-mediated delivery of Luciferase, Beta-galactosidase
(B-gal) and human secreted embryonic alkaline phosphatase (SEAP) marker genes to porcine muscle.
Our patent portfolio in genomics includes GFP (green fluorescent protein) technology, the beta-lactamase platform and certain applications of the beta-galactosidase
The disorder is "caused by mutations in the GLB1 gene, which encodes an enzyme called beta-galactosidase
necessary for recycling of a molecule (GM1-ganglioside) in neurons.
Regulation expression by readthrough translation from a plasmid-encoded beta-galactosidase
Effective reduction of lactose malabsorption and milk intolerance by direct addition of beta-galactosidase
to milk at mealtime.