auxiliary enzyme

auxiliary enzyme

Etymology: L, auxilium, assist
an enzyme that links the enzyme being measured with an indicator enzyme. It is a component of the coupled assay system.
References in periodicals archive ?
This method, which does not need auxiliary enzymes, will also lower reagent costs in contrast to conventional methods that incorporate an auxiliary enzyme.
Methods using various oligosaccharides covalently bound to 2-chloro-4-nitrophenol (CNP) [3] or 4-nitrophenol as substrate and auxiliary enzymes such as [alpha]-glucosidase or glucoamylase for the measurement of total (T-AMY) and pancreatic (P-AMY) amylase activities have been investigated (1-6).
GalG2CNP requires no auxiliary enzymes and is cleaved only at the aglycone bond by amylase.
Method comparisons were performed with sera of various amylase concentrations taken at random using the IFCC method {1(5) substrate 1-[2-chloro-4-nitrophenyl(G1)]-[alpha]-D-maltotrioside (CNP-G3)1 and the test kits [alpha]-Amylase II-A[R] 1(6); substrate, 6-benzyl(G5)-1[4-nitrophenyl(G1)]-[alpha]-D-maltopentaoside; auxiliary enzymes, [alpha]-glucosidase and glucoamylase; Wako} and [alpha]-Amylase EPS[R] [(7); substrate, EPS-G7; auxiliary enzyme, [alpha]-glucosidase from yeast; Boehringer].
As known from the reference method (3), between 25 and 40[degrees]C, the temperature coefficient of Q10 of the auxiliary enzyme (2.
This method can be performed with a minimal volume of sample (1 [micro]L), does not require auxiliary enzymes, catalyst, or sample extraction, and is fast (<5 s per result).
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