Genotyping analysis: Each allele appeared has a peak on electropherogram and as band on autoradiogram
like tiff image file generated by Licor 4300 Genetic Analyzer.
The hippocampal lesions on autoradiograms
were determined accordingly as the % length of hypodense area observed within the Cornu Ammonis (CA) region of the hippocampal stratum pyramidale (100%).
3]R-producing neurons, as shown by the greater binding densities in the representative autoradiograms
of midbrain regions (Figure 6b).
Multiple restriction enzyme digestions (additional to the ones shown), short electrophoresis runs before blotting and overexposures of all autoradiograms
were completed in addition to the results presented above to confirm that all segments of transgenic DNA, including small subfragments, were accounted for in the analysis.
0 of QuantiScan for Windows analyzes images of a wide variety of material including polyacrylamide and agarose gels, autoradiograms
, and TLC plates.
Following densitometric analysis of the Northern blot autoradiograms
, the ratio of FAMTase to 18S rRNA was determined, and the results were displayed graphically (Fig.
As the FBI's leading DNA expert later conceded, there were significant problems when DNA evidence was first introduced in court: "The initial outcry over DNA typing standards concerned laboratory problems: poorly defined rules for declaring a match; experiments without controls; contaminated probes and samples; and sloppy interpretation of autoradiograms
Digital images of the edited autoradiograms
indicating operator determination of band placement were transmitted electronically to the CDC network coordinator for comparison.
Loss of heterozygosity was scored by visual comparison of the intensity of alleles on autoradiograms
For example, an expert's testimony may be that when the DNA fragments on two autoradiograms
are of the same length and in the same position, the match indicates that the fragments may come from the same source, or at least share genetic markers.
They are also used to read chromatograms, protein gels, and autoradiograms
Minisatellites have been a great step forward, but analyses can still be problematic due to overlapping DNA similarity values for some degrees of relatedness and difficulty in scoring across autoradiograms