autoradiogram


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Related to autoradiogram: autoradiography

au·to·ra·di·o·graph

(aw'tō-rā'dē-ō-graf),
Image of the distribution and concentration of radioactivity in a tissue or other substance made by placing a photographic emulsion on the surface of, or in proximity to, the substance.
Synonym(s): autoradiogram

autoradiogram

(ô′tō-rā′dē-ō-grăm′)

autoradiogram

A photograph produced by exposing undeveloped film to a radiolabeled molecule (e.g. DNA, RNA or protein), typically in the context of a restriction enzyme digested blot.
References in periodicals archive ?
Two representative autoradiograms show the length variability of PCR fragment with SSR primers (Fig.
The exposure times of autoradiograms and the intensity of bands may have influenced this determination and the outcome of the matching procedure.
In fact, for all young identified as being illegitimate in this study, we found neighboring males as likely sires (EPF males) and all chicks with two to six diagnostic bands have been rerun and analyzed on a second autoradiogram confirming the results of paternity found in this analysis (Hasselquist et al.
Signal was detected on autoradiograms by chemiluminescence per the manufacturer's instructions (Amersham Life Science).
The autoradiograms from each of the primer sets were scanned into a computer and scored for the presence of AFLP bands that could be clearly resolved in the general range of 60 to 360 bp.
The distilled water eluates contained high amounts of salts, and these autoradiograms showed no well-defined spots.
TRF length estimated by Southern blotting does, however, have disadvantages: (a) TRF does not indicate pure telomere repeat length but includes various unknown lengths of subtelomic sequences; (b) to estimate TRF length, genomic DNA should be purified as intact (unsheared) as possible; (c) the mean TRF length estimated by reading smear patterns of autoradiograms may be inaccurate; and (d) Southern blotting is time consuming.
Autoradiograms were analyzed by quantitative scanning densitometry using a Model GS-700 Imaging densitometer and the Molecular Analyst software (Bio-Rad).
Autoradiograms were produced by exposing Kodak X-Omat AR film to the dried gels for several days at -80 [degrees] C.
The autoradiogram was compared with images downloaded from the soybean database to identify anchor markers on the USDA-ARS-ISU soybean map (SoyBase, 1995).
The cover-slipped sections that were histochemically stained for COX activity and the autoradiograms of Syt4 and Syt7 mRNA levels were transilluminated in the visual field of a 12-bit digital Photometrics camera CoolSNAP cf (Spectra Services Inc.
After 3 days exposure at 4[degrees]C, autoradiograms were washed with D-19 b developer (Kodak) and fixed with Fixaj B (Kodak).