Sputum Smear Fluorescence Microscopy Grading (As per RNTCP)
Auramine O Fluorescent Staining Reporting /Grading Grading (Using 20 or 25x Objective and 10x Eye Piece) > 100 AFB/field after Positive, 3+ examination of 20 fields 11-100 AFB/field after Positive, 2+ examination of 50 fields 1-10 AFB/ field after Positive, 1+ examination of 100 fields Table 1.
5%), when compared with acid fast stain (31%) and
auramine O fluorescent dyes (40.
Sputum samples were screened by fluorescent microscopy using
Auramine O stain and smears found to be positive were confirmed by light microscopy using Ziehl-Neelsen's stain as per the standard protocols of both staining methods.
Smear was prepared and stained with ZN and
auramine.
Kinyoun and Fluorescent staining using
Auramine O and Rhodamine B are better staining techniques in the detection of AFB than Ziehl Neelsen Staining.
2: Drugs and dyes classified as non-genotoxic hepatocarcinogens * by IARC CAS no Name IARC source 492-80-8
Auramine (Vainio, 1992) (4,4'-carbonimidoyl-bis [N,N -dimet-hylaniline]) 144-02-5 Barbital (Vainio, 1992) 637-07-0 Clofibrate (IARC, 1996) 68-89-3 Dipyrone (Vainio, 1992) 97-77-8 Disulfiram (Vainio, 1992) 938-73-8 Ethenzamide (Vainio, 1992) 50-06-6 Phenobarbital (Vainio, 1992) Classification of carcinogenesis CAS no IARC Dir Reg US-EPA NIH Report 67/548/EEC (1) 1272/2008 (3) (NTP, 2014) (1,2) 492-80-8 2B Cat 3, R40 C2 -- -- 144-02-5 -- -- -- -- -- 637-07-0 3 -- -- -- -- 68-89-3 -- -- -- -- -- 97-77-8 3 -- -- -- 938-73-8 -- -- -- -- -- 50-06-6 2B -- -- -- -- Tab.
119) Use: plasticizer Use: intermediate, Class: phenyl emulsifier Class: alcohol carboxylate 22
Auramine hydrochloride Clomiphene citrate [2465-27-2] [50-41-9] (score = 0.
Microscopy of gastric washings for acid-fast bacilli using the
auramine stain was negative.
The presence of mycobacteria in a given sample can be assessed by Ziehl-Neelsen staining followed by light microscopy or
auramine O staining and fluorescence microscopy (Marais et al.
Comparison of Ziehl-Neelsen and
Auramine O staining methods on direct and concentrated smears in clinical specimens.
12,29] Although staining with
auramine O and fluorescence microscopy improves sensitivity by about 10%, [13] overall performance remains suboptimal, meaning that a negative smear does not exclude the diagnosis of TB in HIV co-infected patients.
Constrain in solving Langmuir Hinshelwood kinetic expression for the photocatalytic degradation of
Auramine O aqueous solutions by ZnO catalyst.
