Alkaline phosphatase, acid phosphatase, leucine arylamidase
, and jV-acteyl-P-glucosaminidase (NAGase) were the only enzymes available for analysis.
Tabatabai MA, Garcia-Manzanedo AM, Acosta-Martinez V (2002) Substrate specificity of arylamidase
Plates were read after approximately 24 hours of incubation, at which time rapid biochemical tests, such as coagulase, catalase, and pyrrolidonyl arylamidase
(PYR, Remel, Lenexa, KS), were performed and the identification was released in the EHR.
and valine arylamidase
detected was strong for all strains tested, However, valine and cystine arylamidase
activities were very low or absent in some strains of Lactococcus.
optochin, bile solubility, and pyrrolidonyl arylamidase
Now, Key characters are used for species and subspecies identification include colony morphology, oxygen requirements, coagulase, clumping factor, heat-stable nuclease, hemolysins, catalase, oxidase, alkaline phosphatase, urease, ornithine decarboxylase, pyrrolidonyl arylamidase
, [beta]- galactosidase, acetoin production, nitrate reduction, esculin hydrolysis and aerobic acid production from a variety of carbohydrates including D-trehalose, Dmannitol, D-mannose, D-turanose, D-xylose, D-cellobiose, L-arabinose, maltose, -lactose, sucrose, and raffinose, and intrinsic resistance to novobiocin and polymyxin B .
Because of the broad specificity of the substrates, there has been some confusion concerning nomenclature: Serum aminopeptidase is also called leucine aminopeptidase (LAP, the term we use here), alanine aminopeptidase (AAP), aminopeptidase N, aminopeptidase (microsomal), or arylamidase
The bacteria produced acid from arabinose, glucose, mannose, and xylose, was negative for glycerol fermentation and leucine arylamidase
and positive for rhamnose fermentation.
max] values for rat and human liver microsomal arylamidase
toward CDEPA was calculated using a Lineweaver-Burke double reciprocal plot.
Isolates were identified as GAS on the basis of p-hemolysis, Gram staining, negative catalase test result, positive pyrrolidonyl arylamidase
test result, and agglutination with Lancefield group A antiserum.
All 4 isolates were [alpha]-glucosidase positive and 3 were alanylphenyl-alanyl-proline arylamidase
Only large colony--forming [beta]-hemolytic streptococci were selected and Lancefield grouped by using a Streptococcal Grouping Kit (Oxoid Diagnostic Reagents, Basingstoke, United Kingdora); suspected GAS isolates were tested for pyrrolidonyl arylamidase