APOB

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APOB

A gene on chromosome 2p24-p23 that encodes apolipoprotein B, the main apoliprotein of chylomicrons and low-density lipoproteins, which appears in plasma as two main isoforms: apoB48 (which is synthesised exclusively in the gut) and apoB100 (which is synthesised in the liver).

Molecular pathology
APOB mutations cause hypobetalipoproteinaemia, normotriglyceridemic hypobetalipoproteinaemia, and hypercholesterolaemia due to ligand-defective apoB.
References in periodicals archive ?
Table 1 shows the fasting serum lipid and lipoprotein, plasma lathosterol and apoB-48 concentrations following four weeks of black tea and water ingestion.
We found no effect of chronic black tea ingestion on the plasma concentrations of chylomicrons and chylomicron remnants as indicated by apoB-48 levels in healthy human subjects.
Similarly, chylomicrons and their remnants contain a single molecule of apoB-48.
Although apoB-48 may be recognized by apoB-100 antibodies owing to cross-reactivity, apoB-100 is by far the predominant apoB molecule (>90%) present in plasma, even in the nonfasting state.
In the present study, we hypothesized that, in men, adiponectin would be the adipocytokine most closely associated with changes in TRLs, namely apoB-48 and RLP-cholesterol, and that this association would be independent of body fat compartments and insulin resistance.
Plasma apoB-48 concentrations were measured by a sandwich ELISA using anti-human apoB-48 monoclonal antibodies (designated B-48-151) as reported previously (interassay CV <5%) (18).
ApoB-48 is required for chylomicron production, and apoB-100 is an essential structural component of VLDL and its metabolic products, intermediate-density lipoprotein (IDL) and LDL.
Plasma apoB-48 is a specific marker of chylomicrons and their remnants, and in the fasting state predicts postprandial lipemia (12).
Most attempts to quantify apoB-48 have used analytical electrophoresis [sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)] with densitometric scanning of Coomassie Brilliant Blue-stained gels (2-4).
52 mmol/L, respectively, it is very likely that intermediate-density lipoprotein and LDL, which in mice are also largely remnant-like particles containing apoB-48, also cause interference with the PEGME measurement of HDL-C.
Contribution of apoB-48 and apoB-100 triglyceride-rich lipoproteins (TRL) to postprandial increases in the plasma concentration of TRL triglycerides and retinyl esters.
The chromogenicity and quantitation of apoB-100 and apoB-48 of human plasma lipoproteins on analytical SIDS gel electrophoresis.