4), 1 mM EDTA, 1 mM EGTA, 5 [micro]g/ml aprotinin, 5 [micro]g/ml leupeptin, 5 [micro]g/ml pepstatin A, 5 [micro]g/ml antipain
, 50 mM NaF, 2 mM sodium orthovanadate, 10 mM sodium pyrophosphate, 0.
The cells were then treated with 1 ml of a 1% solution of Triton X-100 (containing 5 [micro]g/ml each of antipain
, pepstatin, and leupeptin as well as 1 mM phenylmethylsulfonyl fluoride) and kept at on ice for 20 min.
0004% w/v each of antipain
, leupeptin, pepstatin A, chymostatin).
The most effective strategies for limiting the duration of DV were, in order, the use of multiple strategies, the administration of antipain
drugs, and the management of physical needs.
7 [micro]g/mL, and antipain
50 [micro]g/mL) at a weight to volume of20:1.
a drug for chronic pain sufferers, works by mimicking--, the body's own antipain