antiglobulin test

Also found in: Dictionary, Thesaurus, Legal, Financial, Encyclopedia.
Related to antiglobulin test: Coombs test, Indirect Antiglobulin Test


an antibody directed against gamma globulin.
antiglobulin test (AGT) Coombs' test.

Coombs test

a test for antibodies, the so-called anti-human globulin test using either the direct or indirect Coombs tests.
Synonym(s): antiglobulin test

antiglobulin test

a test for the presence of antibodies that coat and damage erythrocytes as a result of any of several diseases or conditions. The test can detect Rh antibodies in maternal blood and is used to anticipate hemolytic disease in the newborn. It is also used to diagnose and screen for autoimmune hemolytic anemias and to determine the compatibility of blood types. When exposed to a sample of the patient's serum, the antiglobulin serum causes agglutination if human globulin antibody or its complement is present. Also called Coombs' test.

an·ti·glob·u·lin test

(an'tē-glob'yū-lin test)
Laboratory assessment to detect red blood cell antibodies in patient serum (indirect) or immunoglobulin bound to the surface of the red blood cell (direct).


Robin R.A., English veterinarian and immunologist, 1921–.
Coombs serum - serum from a rabbit or other animal previously immunized with purified human globulin to prepare antibodies directed against IgG and complement. Synonym(s): antihuman globulin
Coombs test - a test for antibodies, the so-called antihuman globulin test, using either the direct or indirect Coombs tests. Synonym(s): antiglobulin test
direct Coombs test - a test for detecting sensitized erythrocytes in erythroblastosis fetalis and in cases of acquired immune hemolytic anemia.
Gell and Coombs reaction - see under Gell
indirect Coombs test - a test routinely performed in cross-matching blood or in the investigation of transfusion reaction.


an antibody to a heterologous antibody, such as goat antihorse IgG antibody, as used in the Coombs test, ELISA, immunofluorescence or other assays.

antiglobulin test
References in periodicals archive ?
This study compared the indirect antiglobulin test (IAT) tube method with column agglutination technology (CAT).
Spherocytes on a peripheral blood smear and a positive direct antiglobulin test are findings that indicate the occurrence of antibody-mediated in vivo hemolysis.
The standard Ortho Clinical Diagnostic transfusion log is a commercially designed, large multicolumn and multi-row log that includes major columns with subdivisions for the date, patient identification, donor identification, blood type and screen, antibody identification, direct antiglobulin test, compatibility testing, component issuance and disposal and with a column for the technologist involved in testing, as well as a column for additional information.
Results of the patient's direct antiglobulin test (DAT) were strongly positive using broad-spectrum reagents and anti-immunoglobulin G (IgG) and C3 antibodies.
Object of the contract consists of 12 n / a package Package 1 - monoclonal reagents ABO systemPackage 2 - monoclonal reagents rhesusPackage 3 - monoclonal reagents for the determination of the Rh antigensPackage 4 - reagent monoclonal anti-KPackage 5 - monoclonal anti-globulin reagent anti-IgGPackage 6 - monoclonal reagents AssembliesPackage 7 - reagent monoclonal anti-IgA AssembliesPackage 8 - human sera antiglobulin test diagnostic AssembliesPackage 9 - diagnostic sera AssembliesPackage 10 - lectinPackage 11 - 30% bovine albuminPackage 12 - two concentrates goat antibodies.
5 mg/dL), a lactate dehydrogenase activity of 693 U/L (RI, 0-248 U/L), and a positive result in a direct antiglobulin test for IgG.
Already approved for patient and donor ABO/Rh testing, antibody screening using a pooled, two-or three-cell screen, and donor ABO and RH confirmation testing, the expanded test menu options include antibody ID-8 and ID-11 cell panels; weak D testing; Rh and Kell phenotyping; cross match; and direct antiglobulin test.
2% of responding institutions in this survey indicated that they routinely perform an antiglobulin test for the weak D phenotype on patients who test negative with anti-D reagents.
Current guidelines for HS diagnosis include family history, splenomegaly, abnormal CBC indices [spherocytes identification; increase in MCHC (mean corpuscular hemoglobin concentration)], reticulocytes, and RDW (red cell distribution width); and decrease in Hb (hemoglobin) and MCV (mean cell volume), a negative direct antiglobulin test, increased bilirubin, and reticulocytosis.
HBOC-21 was added to incompatible mixed blood (type O positive and type A positive) at the same concentrations (up to 65 g/L) and analyzed with the Gamma-clone [R] antiglobulin test (Gamma Biologicals).
Infants at high risk of severe hyperbilirubinemia are those with jaundice in the first 24 hours, predischarge bilirubin levels in the high-risk zone, blood group incompatibility by positive DAT (direct antiglobulin test or the direct Coombs test) or hemolytic disease, gestational age between 35 to 36 weeks, a previous sibling with phototherapy treatment, and cephalohematoma.
1 - Microcards gel allows execution of an indirect antiglobulin test using antiglobulin reagent multi-specific anti-IgG + C3,- Pos.