antiglobulin test

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Related to antiglobulin test: Coombs test, Indirect Antiglobulin Test


an antibody directed against gamma globulin.
antiglobulin test (AGT) Coombs' test.

Coombs test

a test for antibodies, the so-called anti-human globulin test using either the direct or indirect Coombs tests.
Synonym(s): antiglobulin test

antiglobulin test

a test for the presence of antibodies that coat and damage erythrocytes as a result of any of several diseases or conditions. The test can detect Rh antibodies in maternal blood and is used to anticipate hemolytic disease in the newborn. It is also used to diagnose and screen for autoimmune hemolytic anemias and to determine the compatibility of blood types. When exposed to a sample of the patient's serum, the antiglobulin serum causes agglutination if human globulin antibody or its complement is present. Also called Coombs' test.

an·ti·glob·u·lin test

(an'tē-glob'yū-lin test)
Laboratory assessment to detect red blood cell antibodies in patient serum (indirect) or immunoglobulin bound to the surface of the red blood cell (direct).


Robin R.A., English veterinarian and immunologist, 1921–.
Coombs serum - serum from a rabbit or other animal previously immunized with purified human globulin to prepare antibodies directed against IgG and complement. Synonym(s): antihuman globulin
Coombs test - a test for antibodies, the so-called antihuman globulin test, using either the direct or indirect Coombs tests. Synonym(s): antiglobulin test
direct Coombs test - a test for detecting sensitized erythrocytes in erythroblastosis fetalis and in cases of acquired immune hemolytic anemia.
Gell and Coombs reaction - see under Gell
indirect Coombs test - a test routinely performed in cross-matching blood or in the investigation of transfusion reaction.


an antibody to a heterologous antibody, such as goat antihorse IgG antibody, as used in the Coombs test, ELISA, immunofluorescence or other assays.

antiglobulin test
References in periodicals archive ?
Relative sensitivity of direct antiglobulin test, antibody's elution and flow cytometry in the serologic diagnosis of immune hemolytic transfusion reactions.
The results from this investigation indicate that the performance of antenatal titrations by column agglutination technology (CAT) is at least as good as the performance by the indirect antiglobulin test (IAT) tube method.
One of the key tests in helping to distinguish classes of hemolytic anemia is the direct antiglobulin test (DAT).
In a scrutiny by Rosenfield and Ohno, haemoglobin concentration was distinctly lower and bilirubin concentration was distinctly higher among 14% of DAT positive ABO incompatible infants in comparison with a negative antiglobulin test group.
ABBREVIATIONS: UAB = University of Alabama at Birmingham Hospital; DAY = direct antiglobulin test.
Analysis of 1480 cord blood specimens, with special reference to the direct antiglobulin test and to the group O mother.
Supply of diagnostic reagents for serological systems erythrocyte in antiglobulin test - packaged reagents.
3,4) Similarly, automated testing methods are superior to standard tube low-ionic-strength solution (LISS) indirect antiglobulin test (IAT) (tube LISS-IAT) in detecting antibodies of known clinical significance, therefore increasing blood safety for transfused patients.
2% (2087 of 3588) of participants responded that they routinely performed an antiglobulin test for the weak D phenotype on patients who tested negative by direct agglutination with anti-D reagents.
The result of the direct antiglobulin test (DAT) [3] was 2+ (moderately positive) for IgG, and the eluate was nonreactive.