Apoptosis caused by cisplatin treatment was analyzed quantitatively by a FITC Annexin
V Apoptosis Detection kit II (BD Biosciences, Franklin Lakes, NJ, USA) (Chang et al.
The apoptosis in both the conditions was detected using annexin
V-FITC by FACS.
Circulating Gc-globulin colocalizes with both CD44 and annexin
A2 receptors on the cell surface and may use both pathways to enhance C5a-mediated chemotaxis (33).
The gene functions of this cluster are diverse containing among others, Ly6d (lymphocyte antigen 6 complex), a lymphocytic marker; Cstb (cathespin), an intracellular thiol inhibitor; Nqo1 (DT-diaphorase) a two-electron quinone oxidoreductase whose expression is often associated with redox stress; and another member of the annexin
family, others of which are in the cluster shown in Figure 3A; and Krt8 (Keratin 8), commonly associated with Mallory body formation.
staining confirmed a dose-dependent increase in apoptosis caused by exposure to GRN163L.
Moreover, SC increased the number of annexin
V-positive apoptotic bodies and phosphorylated JNK and p38 in AGS cells.
VisEn's Vice President of Applied Biology, said, "We developed an Annexin
red imaging agent, Annexin
-Vivo 750, which targets Annexin
V, a well-characterized biomarker for apoptosis.
For detecting early and late apoptosis in leukocytes, complex methods are required, such as flow cytometric measurements of annexin
V expression in combination with 7-amino-actinomycin D (7AAD) nuclei staining (6).
Program 2: Annexin
and related peptides, for cardiovascular disease.
Phosphatidylserine externalization was measured by Annexin
Hcy in plasma is mainly bound to albumin, but interactions with ceruloplasmin, fibrin, annexin
II, and transthyretin have been also reported (1-4).
The Company intends to temporarily maintain the clinical trial data, intellectual property developed during the program and the existing GMP Annexin
material until October 31, 2004, in an effort to assist companies that may be interested in purchasing the assets of the program.