amplimer

amplimer

OLIGONUCLEOTIDE used as a PRIMER of DNA synthesis in the POLYMERASE CHAIN REACTION.
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An amplimer of 232 bp for AHCYTOEN gene was obtained from both field and reference samples.
Multiplex PCR amplimer conformation analysis for rapid detection of gyrA mutations in fluoroquinolone-resistant Mycobacterium tuberculosis clinical isolates.
A 535bp amplimer was generated with primers MTLa1-F (5'-TGAAAATGAAGACAATGCGA-3') and MTLa1-R (5'CATCTTTTTCTGCTATCAATTC-3') in the presence of MTL type a DNA, and a 615-bp product resulted from primers MTL[alpha]1-F (5'-ATGAATTCACATCTGGAGGC-3') and MTL[alpha]1-R (5'-CTGTTAATAGCAAAGCAGCC-3') in the presence of MTL type [alpha] DNA.
The second dystrophin amplimer group consists of 38 amplicons of symmetric exons [(0,0), (1,1), (2,2)].
The primer set Aca16Sf1010 (5'-TTATATTGACTTGTACAGGTGCT-3') and Aca16Sr1180 (5'-CATAATGATTTGACTTCTTCTCCT-3'), which was designed to give an amplimer of 161 bp, was used for detecting Acanthamoeba DNA (3).
We had difficulty resolving the 99-bp amplimer cut and uncut fragments from the 22 and 23mer PCR primers on 3% agarose, and the 176-bp product was more easily resolved, as was the 118-bp digestion product.
An important result was that, as expected, Inv22 Southern blot patterns (distal and proximal) yielded the same Inv22-specific amplimer (559 bp) without significant differences in relative signal intensities.
5 [micro]M each amplimer, 100[micro]M deoxynucleotide triphosphates, 10 mM Tris-HCl (pH 8.
This is achieved by covalently attaching one primer to the microwell before amplification, thereby allowing colorimetric detection of a solid-bound amplimer carrying a detector tag after washes.
The DHPLC analysts were blind to the nature and number of heterozygotes present in the sample, although a wild-type control for each amplimer was known.
Here we present the integrated system Three-STAR, the amalgam of three techniques using a STAR annealing function amplimer.