amplification

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amplification

 [am″plĭ-fĭ-ka´shun]
the process of making larger, such as the increase of an auditory or visual stimulus, as a means of improving its perception.
DNA amplification artificial increase in the number of copies of a particular DNA fragment into millions of copies through replication of the segment into which it has been cloned, a type of nucleic acid amplification.
gene amplification a process by which the number of copies of a gene is increased in certain cells because extra copies of DNA are made in response to certain signals of cell development or of stress from the environment. In humans this process is seen most often in malignant cells.
nucleic acid amplification amplification of a specific nucleic acid sequence, such as to test for presence of a given virus or bacteria in a sample. Types include DNA amplification, ligase chain reaction, and polymerase chain reaction.

am·pli·fi·ca·tion

(am'pli-fi-kā'shŭn),
The process of making larger, as in increasing an acoustic or visual stimulus to enhance its perception.
[L. amplificatio, an enlarging]

amplification

/am·pli·fi·ca·tion/ (33000) (am″plĭ-fĭ-ka´shun) the process of making larger, such as the increase of an auditory stimulus, as a means of improving its perception.
gene amplification  the process by which the number of copies of a gene is increased in certain cells; in humans it is most often seen in malignant cells.

amplification

(ăm′plə-fĭ-kā′shən)
n.
1. The act or result of amplifying, enlarging, or extending.
2. Physics
a. The process of increasing the magnitude of a variable quantity, especially the magnitude of voltage, power, or current, without altering any other quality.
b. The result of such a process.
3. Genetics The process by which extra copies of a gene or a DNA sequence are formed.

amplification

[am′plifikā′shən]
Etymology: L, amplificare, to make wider
1 (genetic) a process whereby certain targeted sequences of DNA are replicated by the polymerase chain reaction (PCR) in order to produce multiple copies of a single or limited number of genes. Often this refers to increasing the proportion of plasmid DNA relative to the amount of bacterial DNA.
2 the replication in bulk of an entire DNA library. See also polymerase chain reaction. amplify, v.

amplification

An in vivo (as in the fragile X syndrome) or in vitro (as in cloning or PCR) increase in the number of copies of a specific gene or DNA sequence of interest.

amplification

Molecular biology An in vivo–as in the fragile X syndrome or in vitro–as in cloning or PCR ↑ in the number of copies of a specific gene or DNA sequence of interest. See Cloning, DNA amplification, Exon amplification, Extreme amplification, Gene amplification, Linked linear amplification, PCR, PCR amplification of specific alleles, Solid phase amplification.

am·pli·fi·ca·tion

(am'pli-fi-kā'shŭn)
1. The process of making larger, as in increasing an auditory or visual stimulus to enhance its perception.
2. molecular biology Process of increasing the number of nucleic acid copies in a sample to millions within a short period.
[L. amplificatio, an enlarging]

amplification

The production of extra copies of a DNA sequence. These may be within the chromosomal sequence or outside it.

Amplification

A process by which something is made larger. In clotting, only a very few chemicals are released by the initial injury; they result in a cascade of chemical reactions which produces increasingly larger quantities of different chemicals, resulting in an appropriately-sized, strong fibrin clot.
Mentioned in: Hemophilia

amplification

1. the process of making larger, as the increase of an auditory or visual stimulus, as a means of improving its perception.
2. said of a virus means multiplication; replication.
3. in polymerase chain reaction, the synthesis of multiple copies of a particular nucleic acid template sequence.
References in periodicals archive ?
Ribo-SPIA amplification across 6 orders of magnitude provided strong evidence of the linearity and sensitivity of Ribo-SPIA (Fig.
A total of 27 gene products were quantified in each of the samples (before and after the amplification step) by use of PCR primers designed for quantification at different distances from the 3' end of the specific transcripts.
Unlike the T7-based RNA amplification systems (IVT) that generate cRNA products, Ribo-SPIA generates single-stranded DNA products.
Targets prepared by Ribo-SPIA amplification of small samples of total RNA and the NuGEN fragmentation and biotin-labeling system provide highly reproducible gene expression analysis with the Affymetrix GeneChip array.
The linearity and accuracy of gene expression profiling by the Ribo-SPIA method for amplification of small total RNA samples and analysis on GeneChip arrays was further demonstrated with samples to which in vitro-prepared transcripts were added, as described above.
DIFFERENTIAL GENE EXPRESSION ANALYSIS USING THE Ribo-SPIA RNA AMPLIFICATION METHOD
An important advantage offered by the Ovation RNA amplification and target preparation systems is the generation of single-stranded cDNA products that provide a highly specific hybridization target for oligonucleotide arrays in general and GeneChip arrays in particular.
2]) were very similar for the 2 Ribo-SPIA RNA amplification and target preparation methods, as would be expected given the design of the array (biased toward the 3' portion of the transcripts).
Accurate determination of differential gene expression in small samples requires highly reproducible linear RNA amplification or sample preparation.
The linearity of the Ovation RNA amplification system was evaluated by determination of differential gene expression between total RNA samples, UHR (reference human RNA), and human skeletal muscle (each at 20 ng of total RNA).
The results obtained from replicate independent samples (human liver and UHR at 20 ng of total RNA input each) prepared with the Ovation Biotin System amplification and target systems were compared with those obtained by the standard T7-based protocol using higher input of the same total RNA samples.
The Ribo-SPIA isothermal linear RNA amplification systems are highly reproducible and sensitive (5-100 ng of total RNA input), fast, and simple to operate.