ampholyte


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am·pho·ter·ic e·lec·tro·lyte

an electrolyte that can either give up or take on a hydrogen ion and can thus behave as either an acid or a base.
Synonym(s): ampholyte

ampholyte

/am·pho·lyte/ (-līt) amphoteric electrolyte.

ampholyte

(am′fŏ-līt″) [ampho- + (electro)lyte]
A substance that acts as a base or an acid, depending on the pH of the solution into which it is introduced.
ampholytic (-lit′ik) (), adjective
References in periodicals archive ?
9% (by volume) pH 3-10 ampholytes (SERVALYT[R], SERVA).
Each solution was then dried by centrifugation under reduced pressure, after which 180 =L of rehydration buffer (7 mol/L urea, 2 mol/L thiourea, 20 g/L CHAPS, 50 mmol dithiothreitol, and 5 mL/L ampholytes, pH 3-10) was added to the dry sample to solubilize and denature the proteins.
Each serum sample (500 [micro]g of protein) was solubilized in 8 mol/L urea, 40 mL/L CHAPS, 40 mmol/L Tris, 2 mL/L Bio-Lyte 3/10 ampholytes (Sequential Extraction Buffer 2; Bio-Rad) with 5 mmol/L tris(2-carboxyethyl)phosphine reducing agent and incubated for 1 h at room temperature.
variability in gel conductivity, quality of carrier ampholytes, and electroendosmosis) make IEF inherently difficult to standardize.
In addition, sequestering of iron by the ampholytes during IEF might also occur.
5% ampholytes, pI range 3-7, at constant power (15 W) for 1 h at 4 [degrees]C (refrigerated circulating bath).
In reality, the pH gradient created by ampholytes is subject to disturbances by sample constituents that lead to deflections of the lines of equal pH (iso-pH) and produce bands that have been characterized as "frowns" or "smiles".
Urea, Ready-Mix IEF acrylamide/bisacrylamide (29:1 by weight), ammonium persulfate, and N,N,N,N-tetramethylethylenediamine were purchased from Amersham Biosciences, and the ampholytes Servalyt 2-4, 4-6, and 6-8 were purchased from Serva.
Serum containing ~5 [micro]g of total AFP was applied to a mixture of 50 g/L sorbitol, 100 mL/L glycerol, and ampholytes (6.
For example, isoelectric focusing (IEF) in immobilized pH gradients is compared with IEF in carrier ampholytes pH gradient.