3] Nonstandard abbreviations: BD, Becton Dickinson; TNF-[alpha], tumor necrosis factor [alpha]; LPS, lipopolysaccharide; RBC, red blood cells; PBMC, peripheral blood mononuclear cells; MAPK, mitogen-activated protein kinase; IL, interleukin; MAP, multiple analyte panel; CCL, chemokine ligand; MCP, monocyte chemotactic protein; PTX3, pentraxin 3; bDNA, branched DNA; LAL, limulus amoebocyte
Clotting cells and Limulus amoebocyte
lysate: an amazing analytical tool.
lysate test: an aid to the diagnosis in the septic neonate?
The T2Endotoxin assay is a novel method to screen injectable drugs and medical devices for the presence of endotoxins that are produced by gram negative bacteria or fungi, combining the standard Limulus Amoebocyte
Lysate (LAL) reagent with Company's T2MR technology.
All P-Dex preparations were tested for endotoxin contamination using the Limulus amoebocyte
lysate assay (Seikagaku, Tokyo, Japan).
Endotoxin measurements were performed using the Limulus amoebocyte
lysate assay (QCL-1000; Cambrex BioScience Inc.
It is a waste product produced during the preparation of Limulus amoebocyte
lysate (LAL), a clotting agent used worldwide to test for bacterial contamination of pharmaceuticals and implantable medical devices.
All samples were first subjected to assays for endotoxin contamination (Limulus amoebocyte
lysate method), which may stimulate immune cells, of which we found no evidence.
Comparison of the standard pour plate procedure and the ATP and Limulus Amoebocyte
Lysate procedures for the detection of microbial contamination in intravenous fluids.
The Limulus amoebocyte
lysate (LAL) assay for environmental endotoxin levels was adopted as the standard assay of endotoxin detection by the U.
Samples taken before April 2001 were analyzed by the Limulus amoebocyte
lysate (LAL) assay gel clot method from Associates of Cape Cod.
One example of the latter is the production of Limulus amoebocyte
lysate, proteins from the blood of horseshoe crabs, used clinically to detect endotoxins in intravenous fluids.