Conserved domain, GGCTlike Gamma-glutamyl cyclotransferase (GGCT), the synthesis and metabolism of glutathione 65,420 Dia4p protein-ATP binding, serine- TRNA ligase activity (GO Molecular Function), seryl-tRNA aminoacylation
(GO Biological Process) 67,287 Conserved protein Domain family RT_nLTR_like (retrotransposon) 51,829 Conserved protein Domain family RT_nLTR_like (retrotransposon) 73,974 Protein ZDS2; possibly involved in chromatin silencing at rDNA, cell aging, mitotic cell cycling 52,104 Regulation of fatty acid beta-oxidation using acyl-CoA dehydrogenase, mitochondrial respiratory chain complex I assembly 5,799 No description 44,243 Viral capsid protein, controls injection/incorporation of Viral DNA to host.
3243A>G mutation has been shown to lead to reduced levels of the tRNA [sup]Leu (UUR), decrease in aminoacylation
, and absence of the normal modification with 5-taurinomethyl group at the wobble base.
Separately, a 3-chlorotyrosinecharged tRNA containing a CUs anticodon, was prepared by a combination of chemical synthesis and enzymatic ligation, followed by aminoacylation
of 3-chlorotyrosine using an aminoacyl-tRNA synthetase (Fig.
coli, it has been shown that when the rate of tRNA aminoacylation
does not meet the demands of protein synthesis, the ribosome associated RelA is activated (1).
of mutant tRNAs in MELAS but not in MERRF patients.
10:00 RIBOZYME-CATALYZED AMINOACYLATION
FROM ACETYL COA
A tRNA aminoacylation
system for non-natural amino acids based on a programmable ribozyme.
Leu(UUR)] mutation causes aminoacylation
deficiency and concomitant reduced association of mRNA with ribosomes.
Moreover, it is not difficult to imagine that tRNA aminoacylation
activity might also have evolved in the RNA world to regulate RNA replication, perhaps to identify mature genomes in need of replication, or to withdraw genomes from the replicative pool so that they might serve other structural or catalytic functions.
RNA tetraloops as minimalist structures for aminoacylation
Asn], is also formed by transformation of a mischarged tRNA rather than by direct aminoacylation
with asparaginyl-tRNA synthetase.
Species-specific differences in the operational RNA code for aminoacylation