6] Human genes: DMD, dystrophin (muscular dystrophy, Duchenne and Becker types); AMELX, amelogenin
(amelogenesis imperfecta 1, X-linked); AMELY, amelogenin
The gender indicator amelogenin
can be eliminated from the printouts if there is concern about revealing a genotype that is inconsistent with phenotype, as in the rare case of androgen insensitivity syndrome (XY female).
At first glance, amelogenin
looks all wrong for the job of crystal growth.
In the molecular pathology laboratory at Baylor University Medical Center, STR analysis is performed using a commercially available kit for simultaneous amplification of 9 STR loci, as well as the amelogenin
gene on the X and Y chromosomes.
Evidence for regulation of amelogenin
gene expression by 1,25-dihydroxyvitamin [D.
The PowerPlex 18D System is the only product that provides all 13 CODIS loci plus D2S1338, D19S433, Penta E, Penta D and Amelogenin
Table 3: Observed Results Summary Subject CCR5 D1S80 * Amelogenin
rs671 1 (author; male) WT:WT 24:24 106:112bp G:G 2 (male) WT:WT 24:24 106:112bp nd 3 (female) WT:WT 24:6 106bp nd (-) neg control No product No product No product No product WT--wild type allele; bp--base pairs; G--"G" allele; nd--Not determined.
Sexing goat embryos by PCR amplification of X- and Y- chromosome specific sequence of the amelogenin
Genotypes for the 16 tested amelogenin
and STR loci.
Combining this throughput with the improved efficiency of obtaining 16 loci (13 core loci for CODIS plus Amelogenin
Penta D and Penta E) in a single amplification with the PowerPlex 16(TM) reagent system results in a net sample-result-per- day-throughput unparalleled in the industry.
Extracted DNA was amplified for 8 different genetic loci using the Promega PowerPlex (Promega, Madison, Wis) kit and for the amelogenin
The protein, called amelogenin
, is one of two proteins that provide the biological scaffolding around which mineralization occurs during tooth development.