Circoviruses have an ambisense
genome organization with 2 major inversely arranged open reading frames encoding the rolling circle replication initiator protein gene (Rep) and a capsid protein gene (Cap) (1).
Like other tospoviruses, the genome of TZSV is comprised 3 molecules of negative-sense or ambisense
single-stranded RNAs (ssRNAs) that encode 4 structural proteins and 2 non-structural proteins (Dong et al.
The viruses possess a bisegmented, single-stranded RNA genome with ambisense
coding strategy consisting of a small segment coding for the nucleoprotein and glycoprotein and a large (L) segment coding for the RNA-dependent RNA polymerase and matrix protein.
The genome showed an ambisense
organization and 2 major inversely arranged open reading frames encoding the Rep and Cap proteins (Figure 2, panel A).
Analysis of the genome sequence showed characteristics typical of circoviruses, including an ambisense
organization with 2 major inversely arranged ORFs encoding the putative replication-associated (Rep, 303 aa), and capsid (Cap, 270 aa) proteins.
The S segment shows an ambisense
strategy and encodes nucleocapsid protein N and a nonstructural protein (2).
Arenavirus, a bisegmented ambisense
single-stranded RNA virus, is 1 of the viral pathogens responsible for hemorrhagic fever in Africa and South America.
As expected for LCMV, the sequences defined 2 nonoverlapping genes (genes GPC and NP, with 498 and 558 aa, respectively) arranged in ambisense
direction, separated by an intergenic noncoding region, and flanked by 5' and 3' ends.