Insoluble and soluble non-starch polysaccharides (NSPs) were analyzed by gas chromatography (VARIAN, CP-3800, Walnut Creek, CA, USA) as the alditol
acetate derivatives of monosaccharide based on the method developed by Englyst and Hudson (1993), and Theander and Westerlund (1993).
Individual neutral sugars were analyzed as their alditol
acetates by gas-liquid chromatography (GLC) using an HP 5890 SII with FID system, equipped with a capillary column (025 mm [empty set] x 30 m, model B-210, with film 0.
21) studied the feasibility of using 2 variants of the alditol
acetate procedure to analyze agar markers associated with spores of Bacillus anthracis.
Total, soluble and insoluble NSP and their constituent sugars were determined as alditol
acetates by gas-liquid chromatography for neutral sugars, and by a colorimetric method for uronic acids using a modification of the Uppsala method (Theander et al.
4], the monosaccharide alditol
acetates were prepared using the method described by Jones and Albersheim (1972).
GLCMS of partially methylated alditol
acetates was done on a fused silica capillary column (0.
GC-MS of the partially methylated alditol acetates was performed with a split-splitiess injector, used in the split mode and a Supelco fused silica capillary column (30 m x 0.
5 M trifluoroacetic acid, the partially methylated sugar residues were converted to partially methylated alditol acetates by reduction with [NaBD.
The main task for this system is the identification and quantification of partially methylated alditols
acetates originating from plant polysaccharides.
The concentration of monosaccharide sugars as acetylated alditols
was analyzed using a gas chromatograph with deoxygalactose as the internal standard in double tests.
Entries include parent monosaccharides and their derivatives, modified monosaccharides, disaccharides, tri-, oligo-, and polysaccharides, alditols
and cyclitols, nucleosides, glycoside antibiotics, and other glycosides.