Modified aldehyde fuchsin
staining showed that pancreas of the diabetic rats had irregular islet with decreased secretory granules in beta cells, but changes of pancreatic islet in the animals were minimized by administration of extract.
Tissue sections of 5um thickness were obtained to stain with haematoxylin and eosin stain (HandE) and aldehyde fuchsin stain for beta cells and immunohistochemical (IHC) stain using Bandeiraeasimplicifolia (BS)-1 lectinto stain intra-islet capillaries.
For counting beta cells, sections stained with modified aldehyde fuchsin were used.
5, aldehyde fuchsin (AF), periodic acid-Schiff (PAS), KOH/PAS and PAS/AB (pH 2.
5 for acidic glycoconjugates with carboxylated and sulfate esters; aldehyde fuchsin (AF) for glycoconjugates with sulfate; periodic acid (0.
In addition, stained pancreas was washed with Gomori's aldehyde fuchsin for determination of [beta]-cells in Langerhans islets (Gomori, 1950) as described below.
Histological pancreas islets were checked separately in hematoxylin and eosin with aldehyde fuchsin section.