ADAMTS1, -4, -5, -8, -9 and -15 have aggrecanase activity whereas other members do not.
22) When used at dosages below 10 nmol/L, it causes an increased matrix synthesis while preventing matrix breakdown by inhibiting aggrecanase activity and harmful effects of nitric oxide and interleukin-1 (IL-1) on cartilage tissue.
ADAMTS5 is the major aggrecanase
in mouse cartilage in vivo and in vitro.
5) The inhibition of aggrecanase
response was reported to be a consequence of metabolic changes that followed a marked increase in the intracellular glucosamine concentration, the exact mechanisms not yet being fully elucidated.
Relative messenger RNA expression profiling of collagenases and aggrecanases
in human articular chondrocytes in vivo and in vitro.
They discovered that these agents decreased interleukin-1-induced gene expression of several matrix metalloproteinases and aggrecanases
involved in cartilage breakdown.
These include not only the production of metalloproteinases, collagenases, and aggrecanases
that lead to cartilage breakdown in chondrocytes, but also the production of cytokines, such as interleukin-1 (IL-1), tumor necrosis factor (TNF), IL-6, IL-8, and nitric oxide (NO).