agglutination reaction


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agglutination reaction

the formation of an aggregate after the mixing of a soluble antibody with particulate antigen molecules in an aqueous medium. The visible aggregates are formed when specific antibody cross-links the antigens.
References in periodicals archive ?
Regarding the 10% prevalence of ASA (IgG) in infertile men who have no other problems, sperm mixed agglutination reaction (MAR) should be considered as a routine test in the semen analysis of patients with varicocele (23).
The concentration of HA with a dilution 1/64 showed that the agglutination reaction between the protein immunogenic VNN 45 kDa and erythrocytes of Humpback grouper showed levels of sensitivity to concentrations are 0,015625.
It is known that RF affects immunoassays based on the agglutination reaction because RF accelerates nonspecific agglutination via hydrophobic binding.
During the investigation of the assay chemistry reported here, we performed experiments that allow us to comment on the possible mechanism of the agglutination reaction.
A 3-[micro]L aliquot of human serum-based calibrator was mixed with reaction buffer and particle reagent to a total volume of 194 [micro]L; after temperature equilibration for 30 s, the agglutination reaction was initiated by adding 18 [micro]L of either F[(ab').
2 nmol/L in the final mixture) and water diluent were temperature-equilibrated for 65 s, after which the agglutination reaction was initiated by addition of F[(ab').
In some studies, however, the more viscous fluid from SF was treated with hyaluronidase to make the fluid clearer and the agglutination reaction easier to read.
PEGylation effectively masks the Rh(D) antigen, (5,6) therefore by quantifying agglutination reactions with anti-D sera before, during and after subjecting the PEGylated RBCs to each simulated condition above, a standardized determination of dePEGylation can be established.
To evaluate any change in PEGylation, microscopic agglutination reactions were graded, in quadruplicate, on a hemocytometer for each RBC type and each simulated condition.
If the entire skill objective is: "Given unknown whole blood samples, one set of typing reagents, a serofuge, and an agglutination viewer, grade agglutination reactions to 100% accuracy using AABB standards for grading reactions," the breakdown would be:
Thirteen species from 9 families had positive agglutination reactions for antibodies to MG, but all birds tested negative by polymerase chain reaction (PCR).
Automation is not usually used to measure agglutination reactions, but nephelometry in which the amount of light scatter by particles is measured, has been used to measure agglutination and is then called a particle-enhanced immunoassay.