Sodium azide and Kanamycin provide the selective inhibition required whilst aesculin
and iron salts form an indicator system for the presumptive identification of Enteroccoci (http://sms home.
It was incapable of growing in the absence of NaCl and did not produce acid from D-glucose, Dmannose, D-fructose, maltose, D-mannitol, rhamnose, sucrose, D-trehalose, D-galactose, cellobiose, Draffinose, xylose, lactose, ribose, arabinose, D-melezitose, D-salicin and aesculin
The most recent addition to Lab M's Enterococci range, Bile Aesculin Agar, is widely applicable for the isolation and presumptive identification of Enterococci/Group D Streptococci.
Kanamycin Aesculin Azide Agar offers selective isolation and enumeration of Enterococci while Kanamycin Aesculin Azide Broth is an enrichment and isolation medium for Enterococci.
2, and Bile aesculin
agar (Oxoid, Basingstoke, UK) were sterilized by autoclaving (121[degrees]C, 15 min) while Slanetz and Bartley agar (Oxoid, Basingstoke, UK) and KF Streptococcus agar (Merck VWR, Darmstadt) were boiled.
The genus was confirmed by Gram stain, catalase test, bile aesculin
Exotoxin production medium  Enriched egg yolk medium  Sugar fermentation medium  Aesculin
hydrolysis medium  Gelatin medium  Indole medium.
Enterococci are confirmed by demonstrating aesculin
hydrolysis on Kanamycin Aesculin
zooepidemicus by Vitek2, but aesculin
was not hydrolyzed, and D-ribose fermentation was noted, as previously described for S.
The organism exhibited suicidal phenomenon in glucose fermentation broth, was anaerogenic, and positive for Indole production, aesculin
hydrolysis, Arginine hydrolase.
Oxoid Microbact 12L incorporates 12 tests (10 sugar utilisation tests, aesculin
hydrolysis and a rapid haemolysis test) in a convenient identification strip format.
Group two isolates; H1, H3, H6 and H7; rod cells, motile, creamy circular entire raised colonies, utilize organic compounds; glucose, fructose, and can hydrolyze gelatin and Aesculin
, Oxidase positive, optimum NaCl concentration 8%, optimum pH 7, optimum temperature 37[degrees]C.