Lupenone, isolated from Adenophora triphylla, has been shown to inhibit adipocyte differentiation and expression of adipogenic
marker genes through down-regulation of related transcription factors, particularly the PPAR[gamma] gene.
Aging activates adipogenic
and suppresses osteogenic programs in mesenchymal marrow stroma/stem cells: the role of PPAR-gamma2 transcription factor and TGF-beta/BMP signaling pathways.
Association of DNA methylation levels with tissue-specific expression of adipogenic
and lipogenic genes in longissimus dorsi muscle of Korean Cattle.
The differentiation potential of MSCs was evaluated by culturing cells in high-density cultures in either osteogenic or adipogenic
MSCs were cultured in NH OsteoDiff medium (Miltenyi Biotec, Germany) to promote osteoblast differentiation and in NH AdipoDiff medium (Miltenyi Biotec) to promote adipocyte differentiation: in both cases, the medium was changed twice weekly These culture conditions were maintained for 2-3 weeks, and the evaluation of osteogenic or adipogenic
properties was then performed through immunocytochemical staining with alkaline phosphatase or Oil Red 0 (Sigma-Aldrich, USA), respectively, as well as through morphological observations.
differentiation: Cells derived from whole isolates of endometrium were expanded and passaged in DMEM with 10% FBS.
You can use this matrix as an adipogenic
scaffold for stem cells and transplant it into the body where an organ is damaged.
Suppression of the canonical Wnt/ [beta]-catenin signaling upregulates two adipogenic
transcription factors, CCAAT-enhancer-binding protein-a (C/EBP-[alpha]) and peroxisome proliferator response elements (PPAR[gamma]) (38).
Tests showed that MenSCs could differentiate into adipogenic
, chondrogenic, osteogenic, ectodermal, mesodermal, cardiogenic, and neural cell lineages.
Ghrelin increases in these situations have been postulated to provide compensation through orexigenic and adipogenic
This patent covers adipogenic
(fat forming) cells, compositions that induce adipogenesis from hMSCs, as well as the process of fat induction.
His research led to key developments in the field of MSCs, including identifying key conditions for adipogenic
differentiation of MSCs, careful analysis of chondrogenic differentiation and understanding of the intracellular signaling pathway leading to osteogenic differentiation.