A potential weakness of this study is the use of a constitutive
active mutant of ER[alpha] to facilitate crystallography, which Arao suggests might alter the chemical-receptor interactions.
Zhang's team unexpectedly discovered an active mutant form of human Chk1, which is also a non-natural form of this gene.
Remarkably, the research team discovered that when expressed in cancer cells, this active mutant form of Chk1 permanently stopped cancer cell proliferation and caused cell death in petri dishes even without the addition of any chemotherapeutic drugs.
Additionally, we observed that mice possessing constitutively
active mutant (CAM) [[alpha].
2000) also adapted the enzymatic diffusion zone method to select the
active mutant for glucose oxidase activity.
Tarceva (erlotinib) Is Effective In Blocking Downstream Activation of AKT and ERK In Primary Human Tumor Explant Xenografts Containing Constitutively
Active Mutant K-Ras
1a]AR with enhanced green fluorescent protein (EGFP) or a constitutively
active mutant (CAM) [[alpha].
To address this, transgenic mice were generated which over-express the [alpha]lA-AR with enhanced green fluorescent protein (EGFP) or constitutively
active mutant (CAM) [alpha]lA-AR.
To further optimize TNXI's potential industrial utility random mutagenesis using the TNXI V185T-encoding gene as the template was performed and two highly
active mutants 3A2 (V185T/L282P) and 1F1 (V185T/L282P/F186S) were obtained.
By transfection of cells with dominant negative or constitutively
active mutants of cells we will study the effect of the kinases on the activity or phosphorylation of substrates, and on cell growth etc.
Knowles says he expects that running the most
active mutants through more mutation-and-selection cycles should lead to enzymes that show even more activity.