acridine orange


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ac·ri·dine or·ange

(ak'ri-dēn ōr'enj), [C.I. 46005]
3,6-bis(dimethylamino)acridine hydrochloride; a basic fluorescent dye useful as a metachromatic stain for nucleic acids; also used in screening cervical smears for abnormal and malignant cells, where unusual amounts of DNA and RNA occur during proliferation and in tumors (DNA fluoresces yellow to green; RNA fluoresces orange to red).
An immunologic fluorochrome—fluorescent dye—that nonspecifically binds to RNA (red fluorescence), DNA (green fluorescence), proteins, polysaccharides, glycosaminoglycans
Toxicology AO is carcinogenic, causing mutations by intercalating itself within replicating DNA causing insertional or deletional (‘frame shift’) mutations; AO is not routinely used in histologic laboratories

ac·ri·dine or·ange

(ak'ri-dēn awr'ănj)
[CI 46005] A basic fluorescent dye useful as a metachromatic stain for nucleic acids; also used in screening cervical smears for abnormal and malignant cells.
References in periodicals archive ?
Crocin and safranal were purchased from Fluka (Germany), vitamin E (DL-a Tocopherol acetate) from OSVE Pharmaceutical Co (Tehran, Iran), acridine orange from Merck and reticulocyte stain from Sigma-Aldrich.
Acridine orange stock reagent [A]: Prepare in dark-brown, one-gallon container.
The washed catheter pieces were stained with Acridine orange staining solution for 10 min.
To achieve random distribution of cells on the filter, the sample volume was increased to 2 mL with particle-free water (Turley 1993), and approximately 3 drops of acridine orange solution (1 mg/mL) were added to the sample.
Bacterial cell abundance was determined using Acridine Orange direct counts.
After incubating for an hour, a sample of cells was removed from each well and treated with an acridine orange plus ethidium bromide stain and with 1% paraformaldehyde.
Cardiolipin binding on the solid surfaces was detected by the fluorescent dye 10-N-nonyl acridine orange as described above.
The most commonly used fluorescent dyes include DAPI, acridine orange, ethidium bromide, and propidium iodide.
Evaluation of DNA integrity: The spermatozoa of treated rats and the control group were stained by acridine orange dye according to the modified method of Tejada et al.