acid elution

acid elution

The use of buffered HCl to remove (elute) antigens from the surface of red cells in order to identify those antigens capable of producing a haemolytic reaction or agglutination.
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Scientists then remove the adsorbents from the water and the metals are readily extracted using a simple acid elution method.
2) We found that the Rosette and acid elution techniques were effective at detecting a fetomaternal hemorrhage (FMH) requiring additional units of Rh immune globulin (Rhlg; sensitivity 1.
In our view, improving the effectiveness of RhIg dosing would require 2 things: (1) a more accurate calculation of maternal blood volume, such as Dr Ramsey's RhIg Dose Calculator2; and (2) replacement of acid elution FMH quantitation with flow cytometry in all cases in which additional Rh immune globulin doses are indicated by initial Rosette or acid elution test results.
Actually, the fetal cells are resistant to this acid elution and stain a bright pink with the method we use.
2]-MG affinity-chromatography acid elution pattern is shown in Fig.
2]-MG concentration was in fraction 3, indicating that the acid elution caused dissociation of the [[alpha].
1 mol/L acetic acid), wash reagent II (100 mL of ethyl acetate, 100 [micro]L of glacial acetic acid), acid elution reagent (140 mL of hexane, 60 mL of ethyl acetate, 200 [micro]L of glacial acetic acid), and basic elution reagent (98 mL of ethyl acetate, 2 mL of concentrated ammonium hydroxide).
Extraction and spotting solutions for THC metabolites in urine were also prepared according to manufacturer's recommendations: wash reagent I (20% acetic acid), wash reagent II (90 mL of heptane, 10 mL of dichloromethane), kand acid elution reagent (70 mL of hexane, 30 mL of ethyl acetate, and 100 [micro]L of glacial acetic acid).
Five-hundred microliters each of wash solution I, acid elution reagent, and wash solution II are added and aspirated in sequence.
Acid elution reagent (400 ptL) is added to each SPE concentrator (SPEC) extraction column and allowed to spot directly onto the chromatogram for 2 min.