Acyl-CoA Synthetase and Acetyl-CoA Synthetase are enzymes present in the fatty acid metabolism, preserved in D.
Additionally, an increase in the enzymes present in the metabolism of fatty acids, such as Acetyl-CoA Synthetase (ACeCS1) and Acyl-CoA Synthetase (ACSL1), and an induction of oxidative stress were observed.
Acetate freely diffuses to most organs where it is utilized by Acetyl-CoA Synthetase to generate Acetyl-CoA.
Given that a HFD accelerates the lipid metabolism route and increases the Acetyl-CoA Synthetase enzyme, we can suppose an increase in the amount of acetate available in the organism of the flies fed with coconut oil and, in part, this is one of the causes of the reduction in the lifespan, as presented in this study, since many authors report than acetate metabolism plays a critical and important role in aging, because it is regulated by [NAD.
In summary, our study revealed for the first time that flies fed for seven days with a high-fat diet by coconut oil addition (10% and 20%) have a reduced locomotor performance, an increase in Acyl-CoA Synthetase and Acetyl-CoA Synthetase, and, consequently, an increase in the production of reactive species and thiobarbituric acid reactive substances, an increase of HSP83 and MPK2 expression, which generates an oxidative stress situation.