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Hemoglobin Bart hydrops (Hb Bart syndrome) and hemoglobin H (HbH) disease are the two most significant forms of a-thalassemia seen in clinics.
This is first report from Pakistan for the determination of prevalence of various a-thalassemia mutation/deletions with b-thalassemia major patients by using extended panel.
17 The primers for determination of different subsets of a-thalassemia deletion have been extensively described in the literature.
Other considerations are the prevalence of a-thalassemia in the study population and the required information gain.