Since adopting the best practices in 2009, Sarasota Memorial has successfully maintained a hemolysis
rate of less than 1 percent overall across the health system - a rate well below the national average of 5-7 percent.
According to laboratory protocol, a hemolysis
level corresponding to an approximate hemoglobin concentration below 100 mg/dL is judged to be acceptable for testing.
The hemoglobin concentration was measured as a hemolysis
index (HI) (calibrated in milligrams per deciliter hemoglobin) on the Abbott c8000 analyzer.
If the difference between potassium concentrations in samples with a hemolysis index of 0 and those with a hemolysis index of 1 through 10 was not significant, as assessed by using the above formula, then hemolysis was considered not to be clinically significant.
We found clinically significant interference at a hemolysis index value of 3 or greater.
QI have been trying to find a hemolysis control for our COBAS INTEGRA and have been unsuccessful.
A Hemolysis, or the degradation of red-blood cells (RBCs), has long been known to interfere with several analytical test results.
It is possible that an individual department may have a hemolysis
rate comparable to the ED rate; however, total specimen numbers for each department were not captured.
ATP concentration was plotted vs hemoglobin concentration to create a hemolysis
correction curve (Fig.
It is reasonable to expect a hemolysis
rate of less than 2%.
11877984) states that "there is no interference from hemolysis up to a hemolysis
index of 50 (approximate hemoglobin concentration: 50 mg/dL)".
Estimates of hemolysis were made by determining a hemolysis
index (HI) for each sample using a Hitachi 911 instrument: "HI is reported in hemolysis units corresponding to hemoglobin concentration in mg/dL.