Plasma fibronectin is one of the most abundant matrix proteins in the normal liver and is synthesized by hepatocytes.
Sepsis screen in neonates with evaluation of
plasma fibronectin. Pediatr Infect Dis J.
Oscarsson, "Surface-dependent conformations of human
plasma fibronectin adsorbed to silica, mica, and hydrophobic surfaces, studied with use of atomic force microscopy," Journal of Biomedical Materials Research, Part A, vol.
PCL ([M.sub.W]: 80 kD) and human
plasma fibronectin were obtained from Sigma Aldrich (Saint Louis, MO) as well as all reagents used in functionalization.
[20.] Hayashi M, Yamada KM (1983) Domain structure of the carboxyl-terminal half of human
plasma fibronectin. J Biol Chem 258: 3332-3340.
Bovine serum albumin, human
plasma fibronectin, and its proteolytic fragments, including the III1-C polypeptide (anastellin), guinea pig liver tissue transglutaminase (tTG), human fibrinogen, gelatin, pooled normal rabbit serum, were purchased from Sigma.
Homocysteine Binds to Human
Plasma Fibronectin and Inhibits Its Interaction with Fibrin.
Hcy also binds the fibrinbinding domain of
plasma fibronectin in vitro, inhibiting its ability to bind fibrin (24), and binds factor Va in vitro, making it resistant to inactivation by activated protein C (27).
Briefly, 60 [micro]l of rat
plasma fibronectin standard (10-2000 ng/ml) or cell lysates were incubated with an equal volume of rabbit anti-rat fibronectin (R & D systems, Minneapolis, MN) diluted 1:2000 in wash buffer at 4[degrees]C overnight.