Natamycin, also called
pimaricin, is a naturally occurring fungicide produced during fermentation by bacteria found in soil.
Bark pieces, about 2-4mm-wide, were cut from the edge of the lesions and placed on cornmeal agar (CMA) (Sigma-Aldrich Chemie GmbH, Germany) amended with 10mg
pimaricin, 200mg ampicilin, 10mg rifampicin, 10mg benomyl, 25mg pentachloronitrobenzene and 50mg hymexazol (PARBPH) [8].
Amphotericin B, nystatin, and
pimaricin are polyene antibiotics widely used clinically because of their high affinity for ergosterol and relatively low affinity for its mammalian counterpart, thereby limiting their toxicity in mammalian cells (20).
Samples of roots from these grasses were rinsed thoroughly with water, surface sterilized with 50% ethyl alcohol, and then dried with sterile paper towels before being placed onto selective media (PARP - pentachloronitrobenzene ampicillin rifampicin
pimaricin medium) (Jeffers and Martin 1986).