phage display

A method of using genetically modified bacteriophages to make new proteins and replicate selected proteins, enabling the engineering of antibodies and the development of new drugs.

phage display

A technique that fuses peptides to capsid proteins on the surface of a bacteriophage, which allows screening of libraries of phage-displayed peptides for binding to specific ligands. Identification of the DNA sequence of a selected phage identifies its peptide sequence.

phage display

the display of recombinant PEPTIDES/PROTEINS on the surface of a PHAGE, for example M13, so that displayed proteins can be identified by their interaction with other proteins. The technique involves cloning the gene for the protein in a special phage cloning vector, such that the cloned gene is fused with the gene for a phage coat protein. The gene fusion directs synthesis of a hybrid coat protein, when the vector is propagated in an appropriate bacterial host. Upon assembly of the phages, this protein is inserted into the phage coat and hence displayed on the surface of phage particles. The procedure normally involves the use of PHAGE DISPLAY LIBRARY and rapid screening and selection of desired proteins. Uses include the engineering of a wide range of specific ANTIBODIES.

Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005

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References in periodicals archive

This service, combined with the first level phage display antibody construction and screening techniques, holds the capability of enhancing 10-100 fold on aspects of affinity.

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Sir Greg Winter is a pioneer in monoclonal antibodies; in 2018, he was awarded a Nobel Prize in chemistry for the invention of the technology underpinning our proprietary phage display screening platform that we use to identify Bicycles.

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In 2018, a team of scientists won the Nobel Prize for their work on phage display in the directed evolution of new proteins -- in particular, for the production of antibody therapeutics.

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Even if phage products themselves are products of nature, methods of producing modified phages may be patent eligible if they claim more than an application of a law or product of nature using "well-understood, routine, conventional activities] already engaged in by the scientific community." (200) However, the use of genetic engineering, phage display, and directed evolution methodologies are already common practices for modifying phages, making such method claims dubious under both [section] 101 and [section] 103.

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"Antibodies evolved using a method called phage display can combat autoimmune diseases and in some cases cure metastatic cancer."

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George Smith deserved the quarter of the prize in chemistry for his work, and method, which, in 1985, resulted in developing a method known as phage display, where a bacteriophage--a virus that infects bacteria--can be used to evolve new proteins.

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Since then, antibody engineering technologies have evolved, through mouse--human chimeras, fully humanized antibodies, phage display, and single-chain antibodies, such as those derived from camels and sharks.

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