bungeanum leaves; (2) to measure the antioxidant and antimicrobial activities of the different polarity fractions; (3) to quantify the content of twelve natural compounds (chlorogenic acid, epicatechin, rutin, hyperoside, trifolin, quercitrin, kaempferol-3-rhamnoside, quercetin, nitidine chloride, chelerythrine, xanthyletin, and sesamin) in the different polarity fractions by RP-HPLC analysis; and (4) to compare the similarities and differences of the phytochemical composition in the different polarity fractions.
Louis, uSa); vanillin, bromocresol green, tetrahydrofuran (THF), sodium borohydride, and trifluoroacetic acid (Chengdu Kelong Chemical Co., Ltd., China); chloranil (Aladdin Industrial Corporation, Shanghai, China); gallic acid, chlorogenic acid, epicatechin, rutin, hyperoside, trifolin, quercitrin, kaempferol-3-rhamnoside, quercetin, nitidine chloride, chelerythrine, xanthyletin, and sesamin (Shanghai Winherb Medical Science Co., Ltd.); and amphotericin and benzylpenicillin (Shanghai Sunny Biotechnology Co.
The content of twelve compounds (chlorogenic acid, epicatechin, rutin, hyperoside, trifolin, quercitrin, kaempferol-3-rhamnoside, quercetin, nitidine chloride, chelerythrine, xanthyletin, and sesamin) was assayed using an Agilent Technologies 1260 series liquid chromatograph (RP-HPLC) coupled with a variable wavelength detector.
Epicatechin (27.45 mg/g), rutin (16.86 mg/g), hyperoside (19.25 mg/g), quercitrin (16.73 mg/g), chlorogenic acid (3.78 mg/g), kaempferol-3-rhamnoside (3.75 mg/g), trifolin (4.53 mg/g), and sesamin (5.13 mg/g) were the major phenolic components in ECE, other compounds (quercetin, nitidine chloride, chelerythrine, and xanthyletin) had a lower level of content (less than 1 mg/g).
Among the five subfractions, peaks 1-9 (chlorogenic acid, epicatechin, rutin, hyperoside, trifolin, quercitrin, kaempferol-3-rhamnoside, quercetin, and nitidine chloride) were common peaks in EAF and AF.