Reduced glutathione (GSH), 1-chloro-2,4-dinitrobenzene (CDNB), reduced
nicotinamide adenine dinucleotide phosphate (NADPH), amidopyrine, cytochrome C, thiobarbituric acid and all other chemicals were purchased from Sigma Chemical Company (Saint Louis, USA).
G6PD catalyses
nicotinamide adenine dinucleotide phosphate (NADP) to its reduced form NADPH.
(1) G6PD catalyzes the first oxidation reaction in the pentose phosphate shunt, in which glucose-6-phosphate is dehydrogenated to 6-phosphogluconolactone with the concurrent reduction of
nicotinamide adenine dinucleotide phosphate (NADP).
Utilization of oxygen and reduces
nicotinamide adenine dinucleotide phosphate by human placental microsomes during aromatization of androstenedione.
The measurement is based on the fact that the enzymatic reduction of diacetyl requires the cofactor
nicotinamide adenine dinucleotide phosphate (NADPH).
Superoxide anion production secondary to the assembly of the PMN
nicotinamide adenine dinucleotide phosphate (reduced form; NADPH) oxidase is a standard measure of PMN activation.