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Effects of tissue fixatives on antigen preservation for immunohistochemistry: a comparative study of microwave antigen retrieval on Lillie fixative and neutral buffered formalin.

Standardization of Diagnostic Immunohistochemistry: Literature Review and Geisinger Experience

Commercially available primary monoclonal antibody against CD20 (clone UCHT1, Dako) was used to stain 3.0 [micro]m sections of liver biopsies after deparaffinization by routine microwave antigen retrieval and horseradish peroxidase (diaminobenzidine) kit (DAKO, Carpinteria, CA), following manufacturer's instructions.

Clinical feature of intrahepatic B-lymphocytes in chronic hepatitis B

One or more of the following antibodies were used after microwave antigen retrieval: Cytokeratin AE1/AE3 (CK AE1/AE3, Dako, Germany, prediluted); CD31 (Dako, Germany, 20:1000); CD34 (Dako, Germany, 10:1000); Factor VIII (Dako, Germany, 20:1000); CD68 (Dako, Germany,10:1000); and Epithelial Membrane Antigen (EMA, Dako, Germany, 5:1000).

Malignant vascular tumours associated with the breast: a study of 7 cases

Well-controlled immunohistochemical stains for CK and EMA (DakoCytomation, Carpinteria, Calif) were performed on all cases with sufficient histologic material according to established protocols using the diaminobenzidine detection with microwave antigen retrieval in a citrate buffer.

Cytokeratin and epithelial membrane antigen expression in angiosarcomas: an immunohistochemical study of 33 cases

A conventional avidin-biotin complex method was used with microwave antigen retrieval. Appropriate positive and negative control slides were stained in parallel.

Obliterative muscularization of the small bowel submucosa in crohn disease: a possible mechanism of small bowel obstruction

Microwave antigen retrieval was used with cytokeratin and Bcl-2 oncoprotein.

Inflammatory Pseudotumor of the Submandibular Gland: Report of a Case Presenting With Autoimmune Disease-like Clinical Manifestations