microinjection

(mī′krō-ĭn-jĕk′shən)

Injection of minute amounts of a substance into a microscopic structure, such as a single cell.

mi′cro·in·ject′ (-jĕkt′) v.

microinjection

A method of introducing DNA into a cell by direct injection into the nucleus, using a microcapillary pipette.

microinjection

a method for introducing new DNA into a CELL by injecting it directly into the NUCLEUS or CYTOPLASM using a thin glass needle. The technique has been used to inject cloned genes into the nucleus of an egg cell in vitro, to generate a TRANSGENIC ORGANISM.

Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005

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References in periodicals archive

Activity of fCas9 was proven in animals; for instance, mice bearing mutation on the Bcr locus were generated by microinjection of fCas9 and gRNAs into fertilized eggs (22).

An update on the tools for creating transgenic animal models of human diseases--focus on atherosclerosis

A novel system consisting in a mold with inserts and heating elements to control the temperature of the surface of a mold for microinjection molding was developed by De Santis and coworkers [11, 12].

Dynamic Local Temperature Control in Micro-Injection Molding: Effects on Poly(lactic acid) Morphology

Lim et al., "Localized intradermal microinjection of tranexamic acid for treatment of melasma in Asian patients: A preliminary clinical trial," Dermatologic Surgery, vol.

Evaluation of Therapeutic Efficacy and Safety of Tranexamic Acid Local Infiltration in Combination with Topical 4% Hydroquinone Cream Compared to Topical 4% Hydroquinone Cream Alone in Patients with Melasma: A Split-Face Study

As shown in Figures 5(a) and 5(b), microinjection of baclofen into the NTS induced an elevation in BP in varying degrees in different groups.

Electroacupuncture Improves Baroreflex and [gamma]-Aminobutyric Acid Type B Receptor-Mediated Responses in the Nucleus Tractus Solitarii of Hypertensive Rats

The microinjection of saline (100-150 nL, n=10) into the CnF did not affect [DELTA]MAP (before: 72.1[+ or -]4.59 mm Hg, after: 76.4 3.3 mm Hg) or [DELTA]HR (before: 324.8[+ or -]6.4 beats/min, after: 336[+ or -]6.2 beats/min).

Role of the nitrergic system of the cuneiform nucleus in cardiovascular responses in Urethane-Anesthetized male rats

Microinjection of full-length and truncated Xpress-Egr3DsRed2 does not hinder oocyte maturation

Expression of Egr3 in mouse gonads and its localization and function in oocytes

The needle was connected to a 20 [micro]L Hamilton syringe placed on a microinjection pump (CMA/100; BAS, Stockholm, Sweden) with a Teflon tube (0.65 mm OD 9, 0.12 mm OI; Bioanalytical Systems Inc., West Lafayette, IN).

Muscarinic Receptors Types 1 and 2 in the Preoptic-Anterior Hypothalamic Areas Regulate Ovulation Unequally in the Rat Oestrous Cycle

Microinjection method: Microinjection method has been used successfully in the production of transgenic fish and is a commonly used technique due to its simplicity and reliability [7].

Importance of transgenic fish to global aquaculture: a review

Another interesting aspect regarding TA reported in literature is its effectiveness as topical as well as localized intradermal microinjection for melasma.202114 As dose of oral TA in melasma is far less than that prescribed for its hemostatic action

Oral tranexamic acid in treatment of melasma in Pakistani population: a pilot study

The mRNA format is used for multiplexed screening of gRNA sequences, applications that need delivery by microinjection and easier dosage optimisation of Cas9 protein to gRNA in the cell.

Thermo Fisher Scientific launches GeneArt CRISPR products for genome editing