These consequences led the researchers to look beyond viruses to deliver the molecules and some of them include non-viral methods like the injection of naked DNA,
Lipofection, which can eliminate the potential adverse effects that might be caused due to viral vectors.
[ClickPress, Mon Oct 01 2018] According to the new market research report "Transfection Reagents and Equipment Market by Method (Biochemical (Calcium Phosphate,
Lipofection, Dendrimers), Physical (Electroporation, Nucleofection), Viral)), Application (Biomedical, Protein Production), End User - Global Forecast to 2021" , analyzes and studies the major market drivers, restraints/challenges, and opportunities.
[USPRwire, Mon Oct 01 2018] According to the new market research report "Transfection Reagents and Equipment Market by Method (Biochemical (Calcium Phosphate,
Lipofection, Dendrimers), Physical (Electroporation, Nucleofection), Viral)), Application (Biomedical, Protein Production), End User - Global Forecast to 2021" , analyzes and studies the major market drivers, restraints/challenges, and opportunities.
In the meantime,
lipofection protocols (Liu et al., 2009) and new electroporation technologies including nucleofection (Siemen et al., 2005) have been developed that enable efficient plasmid transfection (Templin et al., 2012) and generation of hPSC lines with more stable transgene expression (Schwanke et al., 2014).
coli were introduced into pig fetal fibroblasts by
lipofection, as described below.
In cultures, CRISPR cargo is commonly delivered by
lipofection, electroporation, nucleofection, and microinjection.
HSA-PEI/pEGFP had been shown to provide better pEGFP transfection efficiency than PEI/pEGFP, jetPEI/pEGFP or
lipofection. In order to evaluate whether the effect of HSA-PEI was related to the albumin receptor or not, A549 cells were treated with HSA, ranging from 1 to 50 mg/mL.