Tel Aviv University researchers, led by Michael Milyavsky, discuss their unique genetically encoded sensor and its ability to identify, isolate and characterize
leukemic stem cells in a study published in January.
We show that
leukemic cells and colon cancer cells grow in hPL equally well to slightly better than in FCS.
After removing the medium from plates, MSCs were adherent and MOLT-4 cells were suspended and in the medium just containing MOLT-4 cells, total RNA from
leukemic cells was extracted using RNX-Plus solution kit (Sinaclon, Tehran, Iran).
The course of disease experienced by this patient suggested that GIT involvement with leukemia might parallel
leukemic disease activity, as acceleration of the patient's WBC coincided with a worsening of his symptoms.
Peripheral smears, bone marrow aspirate, H&E and IHC staining show two distinct populations of
leukemic cells.
In my case, the
leukemic cells had lodged in and overwhelmed my spleen.
This also involved the left C6 to T1 nerve roots and extended into the supraclavicular and retroclavicular segment of the left brachial plexus over approximately 6.8 cm in length, most consistent with
leukemic infiltration.
As described for MDS, in AML, MSC-derived endothelial cells are significantly increased, especially in cases with rapidly proliferating disease, further suggesting MSCs derived cell implication in
leukemic niche building.
Following this, immunohistochemistry was done which showed T cells positive for CD3/CD45RO with the histiocytes immunoreactive for CD68 in upper dermis suggestive of leprosy (Figures 5 and 6) and lower dermis with B-cells immunoreactive for CD20/CD5/CD23 suggestive of
leukemic infiltrate (Figures 7 and 8).