For the diagnosis of the disease and follow up of patients, different laboratory methods are employed like the smear, the biopsy, and the
lepromin. A clinical exam and a good clinical history are vitally important.
The Mitsuda test was read by an experienced leprosy specialist 28 days after an intradermal injection of 0.1 ml
lepromin suspension (6.0 X 107 bacilli/ml, heat killed, supplied by the Instituto Lauro de Souza Lima, Bauru, Sao Paulo, Brazil) in the upper one-third of the anterior aspect of the right forearm.
1) Pregnancy or breast feeding; 2) Smear negative and
lepromin positive [but smear negative and
lepromin negative were included]; 3) History of pulmonary tuberculosis, and/or other systemic conditions such as cancer, diabetes, hypertension, renal, hepatic or cardiac diseases; 4) HIV positivity or symptoms suggestive of HIV infection such as recurrent infection.
leprae bacilli (
lepromin) and is used for classification of leprosy.
Although the majority of armadillos develop a lepromatous form of leprosy, the full spectrum of histopathological responses can be observed among armadillos and the animals can be classified according to the Ridley-Jopling scale from lepromatous (LL) to tuberculoid (TT) using
lepromin skin testing.
(16) The
lepromin test, on the other hand, measures the potential of an individual in building up granulomatous response to the mixture of M.
being
lepromin negative, where as BCG vaccination decreases the risk.
In this timing set, HIV infection before leprosy, cellular immune response represented by
lepromin reaction, lymphoproliferation and INF-gamma release were relatively affected in both lepromatous and tuberculoid forms.
Effect of levamisole therapy on
lepromin reaction in lepromatous leprosy cases.
(2) The Mitsuda test involves an intradermic injection of
lepromin, a suspension of heat-killed M.
(4) These evolving classifications were based on clinical features with some support from histological and prognostic features and
lepromin testing.
A nerve biopsy and
lepromin test would be required to know the exact location of these patients in the spectrum.