Englund, "Drug-promoted cleavage of
kinetoplast DNA minicircles.
The Trypanosoma cruzi Satellite DNA OligoC-TesT and Trypanosoma cruzi
Kinetoplast DNA OligoC-TesT for Diagnosis of Chagas Disease: A Multi-cohort Comparative Evaluation Study.
Electronic microscopy transmission studies with cells treated with pentamidine showed condensation and disruption of
kinetoplast DNA core and collapse of mitochondrial membrane [39, 40].
Detection of Trypanosoma cruzi in blood specimens of chronic chagasic patients by polymerase chain reaction amplification of
kinetoplast minicircle DNA: comparison with serology and xenodiagnosis.
At this magnification they were seen as an oval body with clear nucleus and sometimes a small
kinetoplast. They were not seen in isolation but were always be in a group in the cytoplasm of a macrophage (Figure 1) Statistical analysis The sensitivity and specificity of slit-skin smear
These round forms are mostly without nucleus or
kinetoplast and are devoid of dividing capabilities.
These studies revealed overexpression of metabolically significant proteins like carboxypeptidase, enolase, fructose-1, 6-bisphosphatase, Hsp70, and Hsp83 and downregulation of a
kinetoplast membrane protein (KMP11) and calcineurin in R-strains [29-31].
[35] using the following primers to amplify a fragment of 330 bp: 121 (5'-AAA TAA TGT ACG GG(G/T) GAG ATG CAT GA-3')and 122(5'-GGT TCG ATT GGG GTT GGT GTA ATA TA-3') from a nonvariant region of the
kinetoplast DNA minicircles of T.
Barker, "Diagnosis of new world leishmaniasis: specific detection of species of the Leishmania braziliensis complex by amplification of
kinetoplast DNA," Acta Tropica, vol.
Sensitive detection and schizodeme classification of Trypanosoma cruzi cells by amplification and
kinetoplast minicircle DNA sequences: use in diagnosis of Chagas disease.
Leishmain stain of blood film revealed trypomastigote form of Trypanosoma brucei having long cylinder body, central nucleus and undulating membrane with long flagellum arising from
kinetoplast located at posterior end (Fig 1).