in·ser·tion se·quence

discrete DNA sequences of nucleotides that are repeated at various sites on bacterial chromosomes, certain plasmids, and bacteriophages and that can move from one site to another on the chromosome, to another plasmid in the same bacterium, or to a bacteriophage.

Farlex Partner Medical Dictionary © Farlex 2012

insertion sequence (IS)

a type of TRANSPOSABLE GENETIC ELEMENT that codes for its own transposition but which apparently carries no other genetic information. Generally it is several hundred base pairs in length. It is capable of causing MUTATION when it inserts into a GENE. Insertion sequences may exist as independent entities or may form the terminal parts of certain TRANSPOSONS.

Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005

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References in periodicals archive

Polymerase chain reaction for insertion sequence 6110

Improved diagnostic potential of polymerase chain reaction by amplification of multiple gene targets in osteoarticular tuberculosis

20 OXA-48-like] VIMF GTGAGTATCCGACAGTC [bla.sub.VIM-1] 18 VIMR GAGCAAGTCTAGACCG [bla.sub.VIM-1] 18 KPCF TGTCACTGTATCGCCGTC [bla.sub.KPC-1] 21 KPCR CTCAGTGCTCTACAGAAAACC [bla.sub.KPC-1] 21 SPMF CTGCTTGGATTCATGGGCGC [bla.sub.SPM-1] 22 SPMR CCTTTTCCGCGACCTTGATC [bla.sub.SPM-1] 22 Extended-spectrum [beta]-lactamases CTXM1F CGCTTTGCGATGTGCAG [bla.sub.CTX-M-1] 17 CTXM1R ACCGCGATATCGTTGGT [bla.sub.CTX-M-1] 17 SHVC AGAAGGGTTATTCT [bla.sub.SHV] 17 TATTTGTCGC SHVD TCTTTCCGATGCC [bla.sub.SHV] 23 GCCGCCAGTCA DEB ATGAGTAAACTTGGTCTGAC [bla.sub.TEM] 24 3061TEM AGGAAGCAAAGCTGAAAG [bla.sub.TEM] 25 GAATCAAATTTGG Insertion sequence ISEcp1F GCAGGTCTTTTTCTGCTCC [ISE.sub.cpl] 26 transposase ISEcp1R TTTCCGCAGCACCGTTTGC [ISE.sub.cpl] 26 transposase Table 2.

Molecular characterisation and epidemiological investigation of an outbreak of [bla.sub.OXA-181] carbapenemase-producing isolates of klebsiella pneumoniae in south Africa

mallei fliP gene and insertion sequence in GenBank accession NC_006348).

The identification and differentiation between Burkholderia mallei and Burkholderia pseudomallei using one gene pyrosequencing

tuberculosis isolates, the use of insertion sequence IS6110 is the most widely used method.

The Presence and Copy Number of Insertion Sequence IS6110 in Mycobacterium tuberculosis Isolates from Pakistan

microti has been identified with the insertion sequence IS6110 which is considered as specific to the members of the MTB complex (Haddad et al., 2004).

Identification of Bovine and Human Tuberculosis in AFB Positive and Negative Patients

This indicated that the insertion sequence can inhibit the interaction between tobacco 14-3-3s and SPSA in yeast.

TFT1 and TFT10 Most Likely Interact with Sucrose-Phosphate Synthase (SPS) in Tomato

Conventional genotyping of the ACE I/D polymorphism involves PCR, using primers that flank the insertion sequence, and exploits the different migration velocities of I- and D-allele-specific PCR products during electrophoresis in gel matrices (32).

Genotyping of the angiotensin I-converting enzyme gene insertion/deletion polymorphism by the TaqMan method

Other authors have shown that exoU has a different G+C content and is adjacent to a probable insertion sequence and therefore may be acquired by horizontal transfer.

Analysis of the exoS, exoT, and exoU genes in P. aeruginosa cystic fibrosis isolates

The genome sequences to be examined were aligned with the phage- and insertion sequence (IS)-masked chromosome sequence of the STEC O157:H7 strain Sakai (22) using MUMmer (34) to identify conserved regions (cutoff thresholds [greater than or equal to] 98% sequence identity and [greater than or equal to] 1,000-bp alignment length) in each strain and the SNP sites located therein.

Genomic Characterization of [beta]-Glucuronidase-Positive Escherichia coli O157:H7 Producing Stx2a

We extracted DNA from grown colonies after heat inactivation by ultrasonic cell lysis and analyzed the DNA by PCRs targeting insertion sequence (IS) 1245, IS900, and IS901.

Mycobacterium avium subsp. hominissuis Infection in a Domestic Rabbit, Germany

The main difference between the 2 prophages was an insertion sequence element, a common source of prophage variation (Figure 2).

Evolutionary Context of Non-Sorbitol-Fermenting Shiga Toxin-Producing Escherichia coli O55:H7

IS, insertion sequence; KR, ketoreductase B domain of mycolactone polyketide synthase genes; ND, not detected.

Mycobacterium ulceraos DNA in Bandicoot Excreta in Buruli Ulcer-Endemic Area, Northern Queensland, Australia