Direct
immunofluorescence test for IgG at basement membrane zone was positive.
Indirect
Immunofluorescence Test. The propagated IBDV viruses grew well in bioreactor due to the presence of expected positive signal seen as a green fluorescence within the cytoplasm of infected cells and showing high amount of viral antigen (Figure 7) compared to the isolates grown in tissue culture flasks (Figure 8).
[3] Nonstandard abbreviations: ANC, absolute neutrophil count; NAIN, neonatal alloimmune neutropenia; FNAIT, fetal and neonatal alloimmune thrombocytopenia; HDN, hemolytic disease of the newborn; HNA, human neutrophil antigen; GAT, granulocyte agglutination test; GIFT, granulocyte
immunofluorescence test; MAIGA, monoclonal antibody-specific immobilization of granulocyteantigen; PCR-SSP, PCR with sequence-specific primers; HNA-1, HNA system 1.
In all the patients direct
immunofluorescence test revealed IgG/C3 linear deposits along BMZ.
Immunofluorescence Test. For the conduct of the
immunofluorescence test, the leukocyte samples inoculated into the 24-well microplates were retrieved from the deep freezer in which they were stored and thawed.
Using indirect
immunofluorescence test antisperm antibodies were detected in the serum of 64.4% of the infertile women (64.4%).
To search for antibodies against SFG rickettsiae, the indirect
immunofluorescence test was done, using the R.
Immunofluorescence test for sensitive detection of varicella-zoster virus-specific IgG: an alternative to fluorescent antibody to membrane antigen test.
Grossi et al., "The burden of the variability introduced by the HEp-2 assay kit and the CAD system in ANA indirect
immunofluorescence test," Immunologic Research, pp.
Direct
immunofluorescence test, electron microscopy and gene mutation studies could not be done due to limited resources.
(1,2) However, in approximately 1% of patients affected by autoimmune disease, especially SS and PM/DM, the ANA indirect
immunofluorescence test can yield negative results, and so a well-founded clinical suspicion could justify the request for the higher level tests.